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ZIRC
ZFIN ID: ZDB-FIG-161227-18
Cortes et al., 2015 - Accumulation of the Vitamin D Precursor Cholecalciferol Antagonizes Hedgehog Signaling to Impair Hemogenic Endothelium Formation. Stem Cell Reports   5:471-9 Full text @ Stem Cell Reports
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Prim-25

Fig. 1

Vitamin D Metabolites Have Differential Effects on HSC Formation

(A) Exposure to D3 (50 μM) decreased runx1/cmyb expression by WISH at 36 hpf, and 1,25(OH)D3 (10 μM) increased expression. Scale bar, 100 μm.

(B) Qualitative phenotypic distribution of embryos from (A) scored with low, medium, or high runx1/cmyb expression in the AGM (n > 50 embryos/condition).

(C) Runx1:GFP embryos exhibited either diminished or enhanced expression with D3 or 1,25(OH)D3 exposure, respectively. Scale bar, 100 μm.

(D) FACS analysis of double-positive HSPCs in Lmo2:dsRed/cMyb:GFP embryos confirmed a 25% (p = 0.014) decrease with D3 versus a 20% (∗∗p = 0.029) increase with 1,25(OH)D3 compared to controls (5 embryos/sample × 4 replicates/condition). Error bars, mean ± SD.

(E) D3 treatment during hemogenic niche specification (12–24 hpf) or HSC induction (24–36 hpf) only decreased runx1/cmyb by WISH in the early exposure window, whereas 1,25(OH)D3 increased expression during either treatment period. Scale as in (A).

(F) Qualitative phenotypic distribution of embryos from (E) scored for runx1/cmyb as in (B) (n > 20 embryos/condition).

See also Figure S1.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: calcitriol Prim-25 ventral wall of dorsal aorta has extra parts of type hematopoietic multipotent progenitor cell, abnormal
Prim-25 ventral wall of dorsal aorta has extra parts of type hematopoietic stem cell, abnormal
WT chemical treatment by environment: calciol Prim-25 ventral wall of dorsal aorta has fewer parts of type hematopoietic multipotent progenitor cell, abnormal
Prim-25 ventral wall of dorsal aorta has fewer parts of type hematopoietic stem cell, abnormal
zf169Tg; zf73Tg chemical treatment by environment: calcitriol Prim-25 ventral wall of dorsal aorta has extra parts of type hematopoietic multipotent progenitor cell, abnormal
Prim-25 ventral wall of dorsal aorta has extra parts of type hematopoietic stem cell, abnormal
zf169Tg; zf73Tg chemical treatment by environment: calciol Prim-25 ventral wall of dorsal aorta has fewer parts of type hematopoietic multipotent progenitor cell, abnormal
Prim-25 ventral wall of dorsal aorta has fewer parts of type hematopoietic stem cell, abnormal
zf188Tg chemical treatment by environment: calcitriol Prim-25 ventral wall of dorsal aorta has extra parts of type hematopoietic multipotent progenitor cell, abnormal
Prim-25 ventral wall of dorsal aorta has extra parts of type hematopoietic stem cell, abnormal
zf188Tg chemical treatment by environment: calciol Prim-25 ventral wall of dorsal aorta has fewer parts of type hematopoietic multipotent progenitor cell, abnormal
Prim-25 ventral wall of dorsal aorta has fewer parts of type hematopoietic stem cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Stem Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Stem Cell Reports