ZFIN ID: ZDB-FIG-161221-68
Matsuoka et al., 2016 - Radial glia regulate vascular patterning around the developing spinal cord. eLIFE   5 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Day 4 to Day 6
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 4 to Day 6

Fig. 5

sflt1 mutants exhibit ectopic venous ISV sprouting around the spinal cord.

(A) Predicted domain structures of WT and mutant Flt1 (mFlt1 and sFlt1). (B) High resolution melt analysis used to determine the flt1bns29 (left panel) and flt1fh390 (right panel) genotypes. Melting curves for 3 independent fish of each genotype are shown. (C–F) Representative brightfield images of 154 hpf flt1+/+ (C), flt1bns29/+(D), flt1bns29/bns29 (E), and flt1fh390/fh390 (F) larvae. Scale bar, 1 mm. (G–I) 154 hpf TgBAC(etv2:EGFP) flt1+/+ (G), flt1bns29/bns29 (H), and flt1fh390/fh390 (I) trunk vasculature. Ectopic vessel sprouting is observed in the dorsal trunk of flt1bns29/bns29 (H), but not flt1+/+ (G) or flt1fh390/fh390 (I), fish. Scale bar, 100 µm. (J) Quantification of average number of somites that showed ectopic blood vessels in flt1+/+, flt1bns29/+, flt1bns29/bns29, and flt1fh390/fh390 larvae (10 somites examined per animal; ≥20 animals examined per genotype). (K–K”) 100 hpf TgBAC(etv2:EGFP);Tg(-0.8flt1:tdTomato) flt1bns29/bns29 trunk vasculature visualized by TgBAC(etv2:EGFP) (K) and Tg(-0.8flt1:tdTomato) (K’) expression. Ectopic vessel sprouts in flt1bns29/bns29 fish derive from venous ISVs in most cases (yellow arrows). Scale bar, 50 µm. (L–L”) High magnification images of 154 hpf TgBAC(etv2:EGFP);Tg(-0.8flt1:tdTomato) flt1bns29/bns29 trunk vasculature. Ectopic vessels in the dorsal trunk exhibit weak Tg(-0.8flt1:tdTomato) expression as vISVs do (yellow arrows, L”). Scale bar, 50 µm. In all panels, values represent means ± SEM (*** indicates p<0.001 by one-way ANOVA followed by Tukey’s HSD test).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ci1Tg control Day 6 trunk vasculature IFL
ci1Tg ; flt1bns29/bns29 control Day 6 trunk vasculature IFL
ci1Tg ; flt1bns29/bns29 ; hu5333Tg standard conditions Day 4 intersegmental artery IFL
Day 4 intersegmental vein IFL
Day 4 intersegmental vein vascular sprouts IFL
Day 4 trunk vasculature IFL
Day 6 intersegmental artery IFL
Day 6 intersegmental vein IFL
Day 6 intersegmental vein vascular sprouts IFL
Day 6 trunk vasculature IFL
flt1fh390/fh390 ; ci1Tg control Day 6 trunk vasculature IFL
RFP ci1Tg ; flt1bns29/bns29 ; hu5333Tg standard conditions Day 4 intersegmental artery IFL
Day 4 intersegmental vein IFL
Day 4 intersegmental vein vascular sprouts IFL
Day 6 intersegmental artery IFL
Day 6 intersegmental vein IFL
Day 6 intersegmental vein vascular sprouts IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ci1Tg ; flt1bns29/bns29 control Day 6 trunk vasculature dorsal region has extra parts of type blood vessel, abnormal
Day 6 trunk vasculature dorsal region has extra parts of type vascular sprouts, abnormal
ci1Tg ; flt1bns29/bns29 ; hu5333Tg standard conditions Day 4 intersegmental vein vascular sprouts increased amount, abnormal
Day 6 intersegmental vein vascular sprouts increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife