FIGURE

Fig. 6

ID

ZDB-FIG-160929-19

Publication

Song et al., 2016 - Arl13b Interacts With Vangl2 to Regulate Cilia and Photoreceptor Outer Segment Length in Zebrafish

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Fig. 6

Suppression of arl13b and PCP components result in shortened photoreceptor cilia and outer segments. (A-D) Immunohistochemistry using antibodies against acetylated α-tubulin (red) to label cilia in retinal cryosections of 5 dpf zebrafish larvae. (E-H) Immunohistochemistry using rhodopsin antibodies to label photoreceptor outer segments in retinal cryosections of 5 dpf larvae. (I) Quantification of cilia length. (J) Quantification of outer segment area. All sections were counterstained with DAPI. Results are significant (****P < 0.0001) using a 1-way ANOVA with Tukey′s multiple comparisons test correction. Error bars: ± SEM. Scale bar: 10 µm.

Expression Data

Antibodies:	Ab1-rho Ab1-tuba
Fish:	WT arl13b^{hi459Tg/hi459Tg} vangl2^m209/m209 vangl2^m209/m209; arl13b^{hi459Tg/hi459Tg}
Anatomical Terms:	photoreceptor cell photoreceptor cell cilium retinal rod cell photoreceptor outer segment
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	arl13b^{hi459Tg/hi459Tg} vangl2^m209/m209 vangl2^m209/m209; arl13b^{hi459Tg/hi459Tg}
Observed In:	photoreceptor cell photoreceptor cell cilium retinal rod cell photoreceptor outer segment
Stage:	Day 5

Phenotype Detail

Acknowledgments

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