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ZIRC
ZFIN ID: ZDB-FIG-160729-8
Lv et al., 2016 - Synaptic Ribbons Require Ribeye for Electron Density, Proper Synaptic Localization, and Recruitment of Calcium Channels. Cell Reports   15(12):2784-95 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. S2

Expression of Ribeye A, Ribeye B nor CtBP in 5dpf wt and ribeye a Δ10 retina. A, Confocal images of Ribeye A, Ribeye B, and CtBP staining (green) in 5dpf wt zebrafish retina. B, 3X magnification of outer plexiform layer staining in wt 5dpf zebrafish retina. C, 3X magnification of inner plexiform layer staining in wt 5dpf zebrafish retina. D, Confocal images of Ribeye A, Ribeye B, and CtBP staining (green) in 5dpf ribeye a Δ10 mutant fish retina. E, 3X amplification of outer plexiform layer staining in 5dpf ribeye a Δ10 mutant fish retina. Notice the dramatic reduction of Ribeye A staining in ribeye a Δ10 mutant fish. F, 3X magnification of inner plexiform layer staining in 5dpf ribeye a Δ10 mutant fish retina. DAPI (blue) stains nucleus. WT and mutant fish were taken under the same settings for the same antibody. Scale bar is 5 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ctbp2a Ab1-ctbp2a WT standard conditions Day 5 retinal inner plexiform layer IHC
Ab1-ctbp2a Day 5 retinal outer plexiform layer IHC
Ab1-ctbp2a ctbp2aya400/ya400 standard conditions Day 5 retinal inner plexiform layer IHC
Ab1-ctbp2a Day 5 Not Detected retinal outer plexiform layer IHC
ctbp2l Ab2-ctbp2l WT standard conditions Day 5 Not Detected retinal inner plexiform layer IHC
Ab2-ctbp2l Day 5 retinal outer plexiform layer IHC
Ab2-ctbp2l ctbp2aya400/ya400 standard conditions Day 5 Not Detected retinal inner plexiform layer IHC
Ab2-ctbp2l Day 5 retinal outer plexiform layer IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctbp IHC WT standard conditions Day 5 retinal inner plexiform layer
IHC Day 5 retinal outer plexiform layer
IHC ctbp2aya400/ya400 standard conditions Day 5 retinal inner plexiform layer
IHC Day 5 retinal outer plexiform layer
Phenotype Details
Fish Conditions Stage Phenotype
ctbp2aya400/ya400 standard conditions Day 5 retinal inner plexiform layer ab1-ctbp labeling decreased amount, abnormal
Day 5 retinal inner plexiform layer ctbp2a expression decreased amount, abnormal
Day 5 retinal outer plexiform layer ctbp2a expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.