ZFIN ID: ZDB-FIG-160630-28
Lovely et al., 2016 - Bmp signaling mediates endoderm pouch morphogenesis by regulating Fgf signaling in zebrafish. Development (Cambridge, England)   143(11):2000-11 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 5

Blocking Bmp signaling does not alter endodermal cell number. (A-F′,M) Confocal images of sox17:dsred transgenic embryos labeled for cell death at 18 and 26hpf. Similar, low levels of apoptosis are present across all groups (18hpf, f=0, P=1; 26hpf, f=0.3, P=0.6, one-way ANOVA, n=4 embryos). (G-L′,N) Confocal images of sox17:dsred transgenic embryos labeled for cell proliferation at 18 and 26hpf. No differences in the level of proliferating endoderm cells are apparent (18hpf, f=0.2, P=0.8; 26hpf, f=0.1, P=0.8, one-way ANOVA, n=4 embryos). Arrows indicate overlap between the transgene and marker expression. Lateral views, anterior to the left. (O) Total endoderm cell numbers were counted via FACS and no statistically significant differences between 26hpf Dorsomorphin-treated, untreated or DMSO-treated embryos were observed (f=1.8, P=0.2, one-way ANOVA, n=8 samples, 10 embryos per sample). Error bars indicate mean+s.e.m. Scale bar: 50┬Ám.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s903Tg chemical treatment: dorsomorphin 14-19 somites endodermal cell amount, normal
14-19 somites endodermal cell apoptotic process process quality, normal
Prim-5 endodermal cell amount, normal
Prim-5 endodermal cell apoptotic process process quality, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development