ZFIN ID: ZDB-FIG-160630-25
Lovely et al., 2016 - Bmp signaling mediates endoderm pouch morphogenesis by regulating Fgf signaling in zebrafish. Development (Cambridge, England)   143(11):2000-11 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-25 to Day 5

Fig. 2

Blocking Bmp signaling early disrupts endoderm morphology and craniofacial development. (A,B) Whole-mount images of viscerocranium at 5 days post-fertilization (dpf). Cartilage is blue and bone is red. Arrowheads point to missing cartilage elements and asterisks label the ceratobranchial cartilages. (A) No craniofacial defects are present following DMSO treatment. (B) Dorsomorphin (DM) treatment from 10-18hpf causes severe craniofacial defects. Ventral views, anterior to the left. Cartilages: Mc, Meckel′s; Ch, ceratohyal; Cb′s, ceratobranchial; Pq, palatoquadrate; HM, hyomandibular. (C,D) The percentage of viscerocranial defects in embryos treated with Dorsomorphin over multiple time windows. n values for all time points are listed in Table 1. (E,F) Confocal images of control and Dorsomorphin-treated sox17:DsRed embryos. (E′-F′′) Imaris surface renderings of E,F. E′′,F′′ are rotated 45° into the plane of view. (G,H) Confocal images of control or Dorsomorphin-treated sox10:mRFP embryos. Compared with controls (E-E′′,G), Dorsomorphin-treated embryos (F-F′′,H) had severe defects to both endoderm and CNCC morphology. Lateral views (except for E′′,F′′, see above), anterior to the left. P, pouch; PA, pharyngeal arch. Scale bars: 100µm in A; 50µm in E,G.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: dorsomorphin Day 5 ceratobranchial cartilage decreased amount, abnormal
Day 5 ceratohyal cartilage morphology, abnormal
Day 5 chondrocranium morphology, abnormal
Day 5 embryonic viscerocranium morphogenesis disrupted, abnormal
Day 5 Meckel's cartilage morphology, abnormal
Day 5 palatoquadrate cartilage decreased size, abnormal
Day 5 palatoquadrate cartilage shape, abnormal
Day 5 pharyngeal arch cartilage hypoplastic, abnormal
Day 5 pharyngeal system development disrupted, abnormal
s903Tg chemical treatment: dorsomorphin Prim-25 embryonic viscerocranium morphogenesis disrupted, abnormal
Prim-25 pharyngeal pouch hypoplastic, abnormal
Prim-25 pharyngeal system development disrupted, abnormal
vu234Tg chemical treatment: dorsomorphin Prim-25 cranial neural crest cell mislocalised, abnormal
Prim-25 embryonic viscerocranium morphogenesis disrupted, abnormal
Prim-25 pharyngeal arch morphology, abnormal
Prim-25 pharyngeal system development disrupted, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development