ZFIN ID: ZDB-FIG-160607-1
Li et al., 2016 - Ubr3, a Novel Modulator of Hh Signaling Affects the Degradation of Costal-2 and Kif7 through Poly-ubiquitination. PLoS Genetics   12:e1006054 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene: ptch2
Antibody: Ab1-ubr3
Fish: WT, ubr3b1250/+ ; ubr3b1251/+
Anatomical Terms: central nervous system, immature eye, trunk
Stage: 14-19 somites
PHENOTYPE:
Fish: ubr3b1250/+ ; ubr3b1251/+
Observed In: immature eye, optic vesicle epithelium
Stage Range: 5-9 somites to 14-19 somites

Fig. 7

Ubr3 is required for Hh signaling and proper optic vesicle morphogenesis.

(A-B) Lateral views of zebrafish embryos at 18-somites stage after removing the yolks, labeled with anti-Ubr3 antibody. Boxes outline the zebrafish retina, arrow points to central nervous system. The remaining signal in ubr3b1250/b1251 mutant corresponds to non-specific autofluorescence emanating from the yolk and some peridermal cells (arrowheads). (C-D) DIC (Differential Interference Contrast) imaging showing lateral views of 6-somites stage zebrafish embryos. (C) Wild-type optic vesicle (arrowheads) is morphologically visible and characterized by a stratified epithelial thickening. The cavitation seam divides the presumptive retina into dorsal and ventral halves. (D) ubr3 trans-heterozygous mutant optic vesicle (arrow) has morphological defects characterized by disorganized tissue that lacks epithelial morphology. The cavitation seam is disrupted or absent. (G-J) In situ hybridization for ptch2 in wild-type (G, I) and ubr3 mutant (H, J) zebrafish embryos. Note the down-regulation of ptch2 in ubr3 mutant optic vesicles (dotted area). Dorsal views of flat-mounted zebrafish embryos. (G, H) Cross sections through the optic vesicle of embryos shown in G and H, respectively. All the scale bars represent 50┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ptch2 WT standard conditions 14-19 somites immature eye ISH
ubr3b1250/+ ; ubr3b1251/+ standard conditions 14-19 somites immature eye ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-ubr3 IHC WT standard conditions 14-19 somites central nervous system
IHC 14-19 somites immature eye
IHC 14-19 somites trunk
IHC ubr3b1250/+ ; ubr3b1251/+ standard conditions 14-19 somites Not Detected central nervous system
IHC 14-19 somites Not Detected immature eye
IHC 14-19 somites Not Detected trunk
Phenotype Details
Fish Conditions Stage Phenotype
ubr3b1250/+ ; ubr3b1251/+ standard conditions 5-9 somites optic vesicle epithelium disorganized, abnormal
5-9 somites optic vesicle epithelium morphology, abnormal
14-19 somites immature eye ptch2 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.