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ZFIN ID: ZDB-FIG-160602-15
McCarthy et al., 2016 - Pdgfra and Pdgfrb genetically interact during craniofacial development. Developmental dynamics : an official publication of the American Association of Anatomists   245(6):641-52 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-25

Fig. 5

Neural crest cells express both pdgfra and pdgfrb. A-A′′: A 24 hpf fli1:EGFP transgenic embryo labeled for pdgfrb mRNA by means of fluorescence in situ hybridization, anterior is to the left. B-B′′: A 36 hpf fli1:EGFP transgenic embryo and labeled for pdgfrb mRNA by means of fluorescence in situ hybridization, anterior is to the left. C-G′′′: A 12 (C-C′′′), 16 (D-D′′2), 20 (E-E′′′), 24 (F-F′′′), 36 (G-G′′′) hpf embryo stained for both pdgfra and pdgfrb mRNA by means of fluorescence in situ hybridization. The neural crest expresses pdgfra (Eberhart et al., 2008), while pdgfrb is restricted medial and ventral to pdgfra expression at 12 hpf. At 16 hpf, cells singly positive for either pdgfra or pdgfrb are observed within the crest stream (D-D′′, arrow and asterisk, respectively). We also observe cells doubly positive within the stream (Arrowhead). More extensive co-expression of pdgfra and pdgfrb is observed at 20, 24, and 36 hr postfertilization (arrowheads in E-E′′). Scale bar = 20 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg standard conditions Prim-5 cranial vasculature IHC
Prim-5 neural crest IHC
Prim-25 cranial vasculature IHC
Prim-25 neural crest IHC
pdgfra WT standard conditions 5-9 somites cranial neural crest ISH
14-19 somites cranial neural crest ISH
20-25 somites cranial neural crest ISH
Prim-5 cranial neural crest ISH
Prim-25 pharyngeal arch cranial neural crest cell ISH
pdgfrb WT standard conditions 5-9 somites cranial neural crest ISH
14-19 somites cranial neural crest ISH
20-25 somites cranial neural crest ISH
Prim-5 cranial neural crest ISH
Prim-25 pharyngeal arch cranial neural crest cell ISH
y1Tg standard conditions Prim-5 cranial vasculature ISH
Prim-5 pharyngeal arch cranial neural crest cell ISH
Prim-25 cranial vasculature ISH
Prim-25 pharyngeal arch cranial neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.