ZFIN ID: ZDB-FIG-160412-21
Baek et al., 2016 - Omeprazole inhibits melanin biosynthesis in melan-a cells and zebrafish. Experimental dermatology   25(3):239-41 Full text @ Exp. Dermatol.
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 2

The inhibitory effects of omeprazole on melanogenesis in zebrafish. (a), OMP inhibited pigment formation in developing zebrafish larvae. Bar: 625 µm. (b), OMP decreased the pigment area density in zebrafish larvae. (c), The intracellular Tyr activity decreased to 48% compared with that of the untreated zebrafish larvae. (d), OMP decreased the total melanin pigment amount in zebrafish larvae. (d), OMP inhibited the expression of TYP, TRP-1a, TRP-2 and MITFb mRNA levels in a dose-dependent manner as detected by real-time RT-PCR analysis. Fold changes relative to each gene level in the control are presented as mean ± SD. Each value is expressed as the mean ± SD of triplicate determinations. *P < 0.05 and **P < 0.01 versus the control group.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mitfb WT chemical treatment by environment: omeprazole Long-pec whole organism RTPCR
tyr WT chemical treatment by environment: omeprazole Long-pec whole organism RTPCR
tyrp1a WT chemical treatment by environment: omeprazole Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: omeprazole Long-pec whole organism tyrp1a expression decreased amount, abnormal
WT chemical treatment by environment: omeprazole Long-pec whole organism mitfb expression decreased amount, abnormal
WT chemical treatment by environment: omeprazole Long-pec whole organism tyr expression decreased amount, abnormal
WT chemical treatment by environment: omeprazole Protruding-mouth pigmentation decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Experimental dermatology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Exp. Dermatol.