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ZFIN ID: ZDB-FIG-160404-33
Houbrechts et al., 2016 - Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function. Molecular and Cellular Endocrinology   424:81-93 Full text @ Mol. Cell. Endocrinol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 6 Effects of deiodinase KD on relative rod and cone photoreceptor number. (A-L) Immunohistochemical stainings with Zpr3, Zpr1, anti-blue opsin and anti-UV opsin antibodies on cryosections of the eye of control (A,D,G,J), D1D2MO (B,E,H,K) and D3bMO (C,F,I,L) larvae at 3 dpf. Zpr1 is a marker for both green and red cone photoreceptors, Zpr3 is a marker for rod photoreceptors and anti-blue and anti-UV opsin label the outer segment of blue and UV cone photoreceptors respectively. Blue: DAPI staining of the nuclei; Green: Zpr1/Zpr3+Alexa488 or anti-blue/anti-UV + FITC. (M-P) Data for relative rod/cone number (expressed per retinal surface area) are shown as mean + SEM (n ≥ 5 per group). Groups with no common letter are significantly different (p < 0.05). Data in (N,O,P) followed Gaussian distribution and were analyzed by one-way ANOVA with Tukey post hoc test. Data in (M) did not assume Gaussian distribution and were analyzed by Kruskal-Wallis and Dunn′s post hoc test. SCMO: control group, D1D2MO: KD of D1+D2; D3bMO: KD of D3b.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
arr3a zpr-1 WIK control Protruding-mouth retinal cone cell IHC
zpr-1 Protruding-mouth retinal photoreceptor layer green sensitive photoreceptor cell IHC
zpr-1 Protruding-mouth retinal photoreceptor layer red sensitive photoreceptor cell IHC
zpr-1 WIK + MO1-dio1 + MO1-dio2 standard conditions Protruding-mouth retinal cone cell IHC
zpr-1 Protruding-mouth retinal photoreceptor layer green sensitive photoreceptor cell IHC
zpr-1 Protruding-mouth retinal photoreceptor layer red sensitive photoreceptor cell IHC
zpr-1 WIK + MO1-dio3b standard conditions Protruding-mouth retinal cone cell IHC
zpr-1 Protruding-mouth retinal photoreceptor layer green sensitive photoreceptor cell IHC
zpr-1 Protruding-mouth retinal photoreceptor layer red sensitive photoreceptor cell IHC
opn1sw1 Ab1-opn1sw1 WIK control Protruding-mouth retinal cone cell IHC
Ab1-opn1sw1 Protruding-mouth retinal photoreceptor layer UV sensitive photoreceptor cell IHC
Ab1-opn1sw1 Protruding-mouth UV sensitive photoreceptor cell photoreceptor outer segment IHC
Ab1-opn1sw1 WIK + MO1-dio1 + MO1-dio2 standard conditions Protruding-mouth retinal cone cell IHC
Ab1-opn1sw1 Protruding-mouth retinal photoreceptor layer UV sensitive photoreceptor cell IHC
Ab1-opn1sw1 Protruding-mouth UV sensitive photoreceptor cell photoreceptor outer segment IHC
Ab1-opn1sw1 WIK + MO1-dio3b standard conditions Protruding-mouth retinal cone cell IHC
Ab1-opn1sw1 Protruding-mouth retinal photoreceptor layer UV sensitive photoreceptor cell IHC
Ab1-opn1sw1 Protruding-mouth UV sensitive photoreceptor cell photoreceptor outer segment IHC
opn1sw2 Ab1-opn1sw2 WIK control Protruding-mouth blue sensitive photoreceptor cell photoreceptor outer segment IHC
Ab1-opn1sw2 Protruding-mouth retinal cone cell IHC
Ab1-opn1sw2 Protruding-mouth retinal photoreceptor layer blue sensitive photoreceptor cell IHC
Ab1-opn1sw2 WIK + MO1-dio1 + MO1-dio2 standard conditions Protruding-mouth blue sensitive photoreceptor cell photoreceptor outer segment IHC
Ab1-opn1sw2 Protruding-mouth retinal cone cell IHC
Ab1-opn1sw2 Protruding-mouth retinal photoreceptor layer blue sensitive photoreceptor cell IHC
Ab1-opn1sw2 WIK + MO1-dio3b standard conditions Protruding-mouth blue sensitive photoreceptor cell photoreceptor outer segment IHC
Ab1-opn1sw2 Protruding-mouth retinal cone cell IHC
Ab1-opn1sw2 Protruding-mouth retinal photoreceptor layer blue sensitive photoreceptor cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-3 IHC WIK control Protruding-mouth retinal rod cell
IHC WIK + MO1-dio1 + MO1-dio2 standard conditions Protruding-mouth retinal rod cell
IHC WIK + MO1-dio3b standard conditions Protruding-mouth retinal rod cell
Phenotype Details
Fish Conditions Stage Phenotype
WIK + MO1-dio1 + MO1-dio2 standard conditions Protruding-mouth retinal photoreceptor layer has fewer parts of type retinal cone cell, abnormal
Protruding-mouth retinal photoreceptor layer has fewer parts of type retinal photoreceptor layer blue sensitive photoreceptor cell, abnormal
Protruding-mouth retinal photoreceptor layer has fewer parts of type retinal photoreceptor layer green sensitive photoreceptor cell, abnormal
Protruding-mouth retinal photoreceptor layer has fewer parts of type retinal photoreceptor layer red sensitive photoreceptor cell, abnormal
Protruding-mouth retinal photoreceptor layer has fewer parts of type retinal photoreceptor layer UV sensitive photoreceptor cell, abnormal
WIK + MO1-dio3b standard conditions Protruding-mouth retinal photoreceptor layer has fewer parts of type retinal cone cell, abnormal
Protruding-mouth retinal photoreceptor layer has fewer parts of type retinal photoreceptor layer blue sensitive photoreceptor cell, abnormal
Protruding-mouth retinal photoreceptor layer has fewer parts of type retinal photoreceptor layer green sensitive photoreceptor cell, abnormal
Protruding-mouth retinal photoreceptor layer has fewer parts of type retinal photoreceptor layer red sensitive photoreceptor cell, abnormal
Protruding-mouth retinal photoreceptor layer has fewer parts of type retinal photoreceptor layer UV sensitive photoreceptor cell, abnormal
Protruding-mouth retinal photoreceptor layer has fewer parts of type retinal rod cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular and Cellular Endocrinology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and Cellular Endocrinology, 424, Houbrechts, A.M., Vergauwen, L., Bagci, E., Van Houcke, J., Heijlen, M., Kulemeka, B., Hyde, D.R., Knapen, D., Darras, V.M., Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function, 81-93, Copyright (2016) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.