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ZFIN ID: ZDB-FIG-160215-6
Nagelberg et al., 2015 - Origin, specification and plasticity of the great vessels of the heart.. Current biology : CB   25:2099-2110 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Pec-fin to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Pec-fin to Day 4

Fig. 5

Specification of the Ventral Head Vasculature Requires the Combined Activities of Tcf21 and Nkx2.5

(A-P′) Live images and schematic diagrams of embryos of indicated stages and genotypes carrying the indicated transgenes. Squares in (A), (E), (I), and (M) indicate magnified regions shown in (B), (F), (J), and (N). Numbers indicate PAAs 3–6 in (B), (F), (J), and (N). Arrowheads indicate the HA. Lateral views (first and second column) and ventral views (third, fourth, and fifth column). Anterior is to the left. The scale bar represents 100 µm. Note that the images in (B), (F), (J), and (N) are thresholded using an ImageJ custom-written macro to only show the endothelial cells (see ImageJscripts2.ijm in Data S1).

(Q) Quantification of the contribution of nkx2.5+ cells to the PAAs 3–6 in wild-type (includes tcf21-/+; nkx2.5-/+ and tcf21-/+; nkx2.5-/+ embryos, n = 37), tcf21 mutant (includes tcf21/ nkx2.5-/+ embryos, n = 13), nkx2.5 mutant (includes nkx2.5/ tcf21-/+ embryos, n = 10), and tcf21; nkx2.5 double mutant embryos (n = 10). Individual data points and the mean with the SEM are indicated. See also Figures S2 and S3. In cases involving tcf21; nkx2.5 double mutant embryos where contribution of nkx2.5+ cells to PAAs was ambiguous due to the presence of neighboring dying nkx2.5+ cells, scoring reflects a baseline assumption of nkx2.5+ cell contribution.

(R) Quantification of lumenized PAAs of indicated genotypes at 60 hpf. The sample numbers for each genotype are: wild-type embryos (includes tcf21-/+; nkx2.5-/+ and tcf21-/+; nkx2.5-/+ embryos) n = 19, tcf21 mutants (includes tcf21/ nkx2.5-/+ embryos) n = 5, nkx2.5 mutants (includes nkx2.5/ tcf21-/+ embryos) n = 7, and tcf21; nkx2.5 double mutant embryos n = 3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mCherry fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Pec-fin aortic arch 3 IFL
Pec-fin aortic arch 4 IFL
Pec-fin aortic arch 5 IFL
Pec-fin aortic arch 6 IFL
Pec-fin cranial vasculature IFL
Protruding-mouth cranial vasculature IFL
Protruding-mouth hypobranchial artery IFL
Protruding-mouth pharyngeal vasculature IFL
Day 4 cranial vasculature IFL
Day 4 hypobranchial artery IFL
Day 4 pharyngeal vasculature IFL
nkx2.5vu179/vu179 ; s896Tg ; fb7Tg standard conditions Pec-fin aortic arch 3 IFL
Pec-fin aortic arch 4 IFL
Pec-fin aortic arch 5 IFL
Pec-fin aortic arch 6 IFL
Pec-fin cranial vasculature IFL
Protruding-mouth cranial vasculature IFL
Protruding-mouth hypobranchial artery IFL
Protruding-mouth pharyngeal vasculature IFL
Day 4 cranial vasculature IFL
Day 4 hypobranchial artery IFL
Day 4 pharyngeal vasculature IFL
s896Tg ; fb7Tg standard conditions Pec-fin aortic arch 3 IFL
Pec-fin aortic arch 4 IFL
Pec-fin aortic arch 5 IFL
Pec-fin aortic arch 6 IFL
Pec-fin cranial vasculature IFL
Protruding-mouth cranial vasculature IFL
Protruding-mouth hypobranchial artery IFL
Protruding-mouth pharyngeal vasculature IFL
Day 4 cranial vasculature IFL
Day 4 hypobranchial artery IFL
Day 4 pharyngeal vasculature IFL
s896Tg ; fb7Tg ; tcf21sk1/sk1 ; nkx2.5vu179/vu179 standard conditions Pec-fin aortic arch 3 IFL
Pec-fin aortic arch 4 IFL
Pec-fin aortic arch 5 IFL
Pec-fin aortic arch 6 IFL
Pec-fin cranial vasculature IFL
Protruding-mouth cranial vasculature IFL
Protruding-mouth Not Detected hypobranchial artery IFL
Protruding-mouth pharyngeal vasculature IFL
Day 4 cranial vasculature IFL
Day 4 Not Detected hypobranchial artery IFL
Day 4 pharyngeal vasculature IFL
ZsYellow fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Pec-fin aortic arch 3 IFL
Pec-fin aortic arch 4 IFL
Pec-fin aortic arch 5 IFL
Pec-fin aortic arch 6 IFL
Pec-fin heart IFL
Protruding-mouth heart IFL
Day 4 heart IFL
nkx2.5vu179/vu179 ; s896Tg ; fb7Tg standard conditions Pec-fin aortic arch 3 IFL
Pec-fin aortic arch 4 IFL
Pec-fin aortic arch 5 IFL
Pec-fin aortic arch 6 IFL
Pec-fin heart IFL
Protruding-mouth heart IFL
Day 4 heart IFL
s896Tg ; fb7Tg standard conditions Pec-fin aortic arch 3 IFL
Pec-fin aortic arch 4 IFL
Pec-fin aortic arch 5 IFL
Pec-fin aortic arch 6 IFL
Pec-fin heart IFL
Protruding-mouth heart IFL
Protruding-mouth hypobranchial artery IFL
Protruding-mouth pharyngeal vasculature IFL
Day 4 heart IFL
Day 4 hypobranchial artery IFL
Day 4 pharyngeal vasculature IFL
s896Tg ; fb7Tg ; tcf21sk1/sk1 ; nkx2.5vu179/vu179 standard conditions Pec-fin Not Detected aortic arch 3 IFL
Pec-fin Not Detected aortic arch 4 IFL
Pec-fin Not Detected aortic arch 5 IFL
Pec-fin Not Detected aortic arch 6 IFL
Pec-fin heart IFL
Protruding-mouth heart IFL
Protruding-mouth Not Detected hypobranchial artery IFL
Day 4 heart IFL
Day 4 Not Detected hypobranchial artery IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Pec-fin aortic arch 3 ZsYellow expression decreased amount, abnormal
fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Pec-fin aortic arch 3 blood vessel lumenization decreased occurrence, abnormal
fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Pec-fin aortic arch 4 ZsYellow expression decreased amount, abnormal
fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Pec-fin aortic arch 4 blood vessel lumenization decreased occurrence, abnormal
fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Pec-fin aortic arch 5 ZsYellow expression decreased amount, abnormal
fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Pec-fin aortic arch 5 blood vessel lumenization decreased occurrence, abnormal
fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Pec-fin aortic arch 6 ZsYellow expression decreased amount, abnormal
fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Pec-fin aortic arch 6 blood vessel lumenization arrested, abnormal
fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Protruding-mouth hypobranchial artery detached from lateral dorsal aorta, abnormal
fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Protruding-mouth hypobranchial artery shape, abnormal
fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Day 4 hypobranchial artery detached from lateral dorsal aorta, abnormal
fb7Tg ; s896Tg ; tcf21sk1/sk1 standard conditions Day 4 hypobranchial artery shape, abnormal
nkx2.5vu179/vu179 ; s896Tg ; fb7Tg standard conditions Pec-fin aortic arch 3 ZsYellow expression decreased amount, abnormal
Pec-fin aortic arch 5 blood vessel lumenization decreased occurrence, abnormal
Pec-fin aortic arch 6 blood vessel lumenization decreased occurrence, abnormal
s896Tg ; fb7Tg ; tcf21sk1/sk1 ; nkx2.5vu179/vu179 standard conditions Pec-fin aortic arch 3 ZsYellow expression absent, abnormal
Pec-fin aortic arch 4 ZsYellow expression absent, abnormal
Pec-fin aortic arch 4 blood vessel lumenization decreased occurrence, abnormal
Pec-fin aortic arch 5 ZsYellow expression absent, abnormal
Pec-fin aortic arch 5 blood vessel lumenization arrested, abnormal
Pec-fin aortic arch 6 ZsYellow expression absent, abnormal
Pec-fin aortic arch 6 blood vessel lumenization arrested, abnormal
Protruding-mouth hypobranchial artery absent, abnormal
Protruding-mouth hypobranchial artery ZsYellow expression absent, abnormal
Protruding-mouth hypobranchial artery mCherry expression absent, abnormal
Protruding-mouth hypobranchial artery detached from lateral dorsal aorta, abnormal
Day 4 hypobranchial artery ZsYellow expression absent, abnormal
Day 4 hypobranchial artery absent, abnormal
Day 4 hypobranchial artery mCherry expression absent, abnormal
Day 4 hypobranchial artery detached from lateral dorsal aorta, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.