FIGURE

Fig. 2

ID
ZDB-FIG-160210-15
Publication
Kim et al., 2015 - Cartilage development requires the function of Estrogen-related receptor alpha that directly regulates sox9 expression in zebrafish
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Fig. 2

The structure of craniofacial cartilage is disorganised upon knockdown of esrra.

(A–H) Embryos were injected with either MOctrl or MOesrra and subject to alcian blue staining at 3- or 4 dpf as indicated. Craniofacial cartilage, especially ceratohyal (CH) and ceratobranchial (CB) cartilage, are abnormally developed in MOesrra-injected embryos when compared to control embryos. Note that the structure of neural cranium is well organised although smaller in size in MOesrra-injected embryos (compare C and G with D and H). (I–K) sox10:GPF (I,J) or fli1:GFP (K,L) transgenic embryos were used to confirm the cartilage defects upon knockdown of MOesrra. (MP) Human ESRRa mRNA (hESRRa) was co-injected with MOesrra into 1-cell stage of embryos which were subject to alcian blue staining. Embryos co-injected with hESRRa and MOesrra show partial restoration of CB (asterisk in O) and repress disorientation of CH induced by MOesrra alone (compare O with N). Misexpression of hESRRa at 100 pg does not impair cartilage development (P, see the text for detail). Embryos are shown in ventral view with anterior to the left.

Expression Data
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Day 4

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 4

Phenotype Detail
Acknowledgments
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