ZFIN ID: ZDB-FIG-160129-8
Zheng et al., 2015 - Caffeine reduces hepatic lipid accumulation through regulation of lipogenesis and ER stress in zebrafish larvae. Journal of Biomedical Science   22:105 Full text @ J. Biomed. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Conditions:
Anatomical Term:
Stage: Days 14-20
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Days 14-20

Fig. 5

Effects of caffeine on the expression of selected genes in livers of zebrafish larvae overfed for 20 days. The relative mRNA expression of genes involved in lipid metabolism (a), ER stress (b), inflammatory factors (c), and autophage (d) in control (30 mg/d) and 5 % caffeine group compared with gene expression in model group (180 mg/d) by qRT-PCR. Expression analysis of the selected genes using cDNA prepared from liver of zebrafish larvae (n = 20) in each group. Data is expressed in mean ± SEM, n = 5. Protein expression of ER stress (e) and inflammatory factor (f) was examined by western blot in caffeine treatment and model group zebrafish larvae. * P < 0.05, * *P < 0.01, * * *P < 0.001 by one-way ANOVA

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
acaca WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
acox3 WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
atf6 WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
atg3 WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
atg12 WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
becn1 WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
cd36 WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
cpt1aa WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
ddit3 WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
eif2ak3 WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
ern2 WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
fasn WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
hspa5 WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
il1b WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
nkap WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
pparab WT control Days 14-20 whole organism RTPCR
WT standard conditions Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
pparg WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
srebf1 WT control Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
tnfa WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
ucp2 WT control Days 14-20 whole organism RTPCR
WT unlimited food availability Days 14-20 whole organism RTPCR
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab2-ddit3 IHC WT control Days 14-20 whole organism
IHC WT unlimited food availability Days 14-20 whole organism
IHC WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism
Ab2-il1b IHC WT control Days 14-20 whole organism
IHC WT unlimited food availability Days 14-20 whole organism
IHC WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism
Ab5-hspa5 IHC WT control Days 14-20 Not Detected whole organism
IHC WT unlimited food availability Days 14-20 whole organism
IHC WT unlimited food availability, chemical treatment: caffeine Days 14-20 Not Detected whole organism
Phenotype Details
Fish Conditions Stage Phenotype
WT unlimited food availability Days 14-20 whole organism atg3 expression decreased amount, abnormal
WT unlimited food availability Days 14-20 whole organism fasn expression decreased amount, abnormal
WT unlimited food availability Days 14-20 whole organism atg12 expression decreased amount, abnormal
WT unlimited food availability Days 14-20 whole organism becn1 expression increased amount, abnormal
WT unlimited food availability Days 14-20 whole organism atf6 expression increased amount, abnormal
WT unlimited food availability Days 14-20 whole organism ucp2 expression increased amount, abnormal
WT unlimited food availability Days 14-20 whole organism tnfa expression increased amount, abnormal
WT unlimited food availability Days 14-20 whole organism ab5-hspa5 labeling increased amount, abnormal
WT unlimited food availability Days 14-20 whole organism ab2-il1b labeling increased amount, abnormal
WT unlimited food availability Days 14-20 whole organism cd36 expression increased amount, abnormal
WT unlimited food availability Days 14-20 whole organism il1b expression increased amount, abnormal
WT unlimited food availability Days 14-20 whole organism ern2 expression increased amount, abnormal
WT unlimited food availability Days 14-20 whole organism hspa5 expression increased amount, abnormal
WT unlimited food availability Days 14-20 whole organism ddit3 expression increased amount, abnormal
WT unlimited food availability Days 14-20 whole organism ab2-ddit3 labeling increased amount, abnormal
WT unlimited food availability, chemical treatment: caffeine Days 14-20 whole organism ab2-il1b labeling amount, ameliorated
Days 14-20 whole organism il1b expression amount, ameliorated
Days 14-20 whole organism ab5-hspa5 labeling amount, ameliorated
Days 14-20 whole organism tnfa expression amount, ameliorated
Days 14-20 whole organism ab2-ddit3 labeling decreased amount, abnormal
Days 14-20 whole organism atf6 expression decreased amount, abnormal
Days 14-20 whole organism hspa5 expression decreased amount, abnormal
Days 14-20 whole organism atg3 expression decreased amount, abnormal
Days 14-20 whole organism becn1 expression decreased amount, abnormal
Days 14-20 whole organism atg12 expression decreased amount, abnormal
Days 14-20 whole organism fasn expression decreased amount, abnormal
Days 14-20 whole organism acaca expression decreased amount, abnormal
Days 14-20 whole organism srebf1 expression decreased amount, abnormal
Days 14-20 whole organism eif2ak3 expression decreased amount, abnormal
Days 14-20 whole organism ddit3 expression increased amount, abnormal
Days 14-20 whole organism ucp2 expression increased amount, abnormal
Days 14-20 whole organism acox3 expression increased amount, abnormal
Days 14-20 whole organism ern2 expression increased amount, abnormal
Days 14-20 whole organism cd36 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Biomedical Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Biomed. Sci.