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ZIRC
ZFIN ID: ZDB-FIG-151207-10
Yuan et al., 2015 - CCAAT/enhancer-binding protein α is required for hepatic outgrowth via the p53 pathway in zebrafish. Scientific Reports   5:15838 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 5

The p53 signaling pathway is activated in cebpa-deficient developing liver.

(AB) Quantitative PCR analysis of the expression of cell proliferation and apoptosis-related genes in 72 hpf embryos. Data shown are the mean ± SD, n e 3, *P < 0.05 by student’s t-test. (C,E) WISH assay of lfabp at 72 hpf. Loss of p53 could rescue the hepatic defect in cebpa mutant embryos. Dorsal views with anterior to the top. (D,F) The relative liver area measured in (C,E) respectively. The result shown is fold difference compared with the level (set to 100) detected in control embryos (mean ± SD, n e 3, **P < 0.005 by student’s t-test).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bcl2a WT standard conditions Protruding-mouth whole organism ISH
cebparj31/+ standard conditions Protruding-mouth whole organism ISH
bcl2l1 WT standard conditions Protruding-mouth whole organism ISH
cebparj31/+ standard conditions Protruding-mouth whole organism ISH
fabp1a WT standard conditions Protruding-mouth liver ISH
cebparj31/+ standard conditions Protruding-mouth liver ISH
cebparj31/+ + MO7-tp53 standard conditions Protruding-mouth liver ISH
cebparj31/rj31 standard conditions Protruding-mouth liver ISH
tp53zdf1/zdf1; cebparj31/rj31 standard conditions Protruding-mouth liver ISH
jun WT standard conditions Protruding-mouth whole organism ISH
cebparj31/+ standard conditions Protruding-mouth whole organism ISH
mycb WT standard conditions Protruding-mouth whole organism ISH
cebparj31/+ standard conditions Protruding-mouth whole organism ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cebparj31/+ standard conditions Protruding-mouth liver fabp1a expression decreased distribution, abnormal
Protruding-mouth whole organism mycb expression increased amount, abnormal
Protruding-mouth whole organism bcl2a expression increased amount, abnormal
Protruding-mouth whole organism bcl2l1 expression increased amount, abnormal
Protruding-mouth whole organism jun expression increased amount, abnormal
cebparj31/+ + MO7-tp53 standard conditions Protruding-mouth liver fabp1a expression spatial pattern, ameliorated
cebparj31/rj31 standard conditions Protruding-mouth intrinsic apoptotic signaling pathway by p53 class mediator increased occurrence, abnormal
Protruding-mouth liver fabp1a expression decreased distribution, abnormal
Protruding-mouth liver decreased size, abnormal
Protruding-mouth liver development process quality, abnormal
tp53zdf1/zdf1; cebparj31/rj31 standard conditions Protruding-mouth liver size, ameliorated
Protruding-mouth liver fabp1a expression spatial pattern, ameliorated
Protruding-mouth liver development process quality, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.