FIGURE

Fig. 4

ID
ZDB-FIG-151022-7
Publication
Mateus et al., 2015 - Control of tissue growth by Yap relies on cell density and F-actin in zebrafish fin regeneration
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Mesenchymal cell density and morphology are altered according to the regenerative stage. (A-C) Quantification of average DAPI intensity (in arbitrary units, a.u.) in mesenchymal cells along the PD axis of blastemas at 24hpa (A), 48hpa (B) and 72hpa (C). n=7 sections, 3 fish/condition. (D-F) Representative DAPI-stained longitudinal sections of blastemas at 24hpa (D), 48hpa (E) and 72hpa (F). (G-I) Quantification of average space (a.u.) between mesenchymal cells along the PD axis at 24hpa (G), 48hpa (H) and 72hpa (I). n=9 sections; 3 fish/condition. (J-L) Representative anti-GFP-stained longitudinal sections of ctgfa:eGFP transgenics at 24hpa (J), 48hpa (K) and 72hpa (L). (M) Quantification of average cell aspect ratio of mesenchymal cells at 24, 48 and 72hpa, in which y is the minor axis of the cell and x the major cell axis. A perfect circular shape corresponds to a ratio between y and x of 1. P corresponds to proximal; D to distal regions. ***P<0.0001; two-tailed, non-parametric Mann–Whitney test. n=45 cells/condition; 5 cells randomly selected/image; 9 sections; 3 fish/condition. (N-R) High magnification images of the boxed areas in J-L highlight cell morphology of blastema cells at 24hpa (N); 48hpa proximally (O) and distally (P); and 72hpa proximally (Q) and distally (R). Dashed lines indicate amputation plane. Scale bars: 50µm. Medial blastema areas were considered for all measurements: shadows indicate the s.e.m. for each curve.

Expression Data
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage: Adult

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development