header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-151022-24
Li et al., 2015 - Overlapping Requirements for Tet2 and Tet3 in Normal Development and Hematopoietic Stem Cell Emergence. Cell Reports   12(7):1133-43 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to High-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to High-pec

Fig. 5

Tet2/3 Are Required for HSC Emergence through the Endothelial to Hematopoietic Transition

(A and B) Merged images depicting GFP and mCherry labeling of the vasculature in Tg(kdrl:Ras-mCherry), Tg(kdrl:H2B-EGFP) transgenic larvae at 30 hpf.

(C) Number of EHT events detected between 30 and 46 hpf in tet2/3DM larvae and siblings. Numerical data are presented as the mean ± SEM.

(D) Number of fragmented nuclei observed in the DA of tet2/3DM larvae and their siblings between 30 and 46 hpf. Numerical data are presented as the mean ± SEM.

(E) Sequences from Movie S1 documenting the stepwise emergence of an HSC from the DA of a sibling larva. For each time point, merged GFP and mCherry images are shown. The yellow arrow indicates the cell undergoing EHT.

(F) Sequences from Movie S2 documenting a cell undergoing nuclear fragmentation in the DA of a tet2/3DM larva. For each time point, merged GFP and mCherry images are shown. The yellow arrow indicates the cell with nuclear fragmentation.

All scale bars indicate 30 µM. See also Movies S1 and S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP mu122Tg ; s896Tg standard conditions Prim-15 dorsal aorta nucleus IFL
Prim-15 vascular endothelium nucleus IFL
Prim-15 to High-pec dorsal aorta hematopoietic stem cell IFL
Prim-15 to High-pec dorsal aorta nucleus IFL
Prim-15 to High-pec vascular endothelium nucleus IFL
tet2mk17/mk17 ; s896Tg ; tet3mk18/mk18 ; mu122Tg standard conditions Prim-15 dorsal aorta nucleus IFL
Prim-15 vascular endothelium nucleus IFL
Prim-15 to High-pec dorsal aorta hematopoietic stem cell IFL
Prim-15 to High-pec dorsal aorta nucleus IFL
Prim-15 to High-pec vascular endothelium nucleus IFL
mCherry mu122Tg ; s896Tg standard conditions Prim-15 dorsal aorta membrane IFL
Prim-15 dorsal aorta ventral region IFL
Prim-15 vascular endothelium membrane IFL
Prim-15 to High-pec dorsal aorta hematopoietic stem cell IFL
Prim-15 to High-pec dorsal aorta membrane IFL
Prim-15 to High-pec dorsal aorta ventral region IFL
Prim-15 to High-pec vascular endothelium membrane IFL
tet2mk17/mk17 ; s896Tg ; tet3mk18/mk18 ; mu122Tg standard conditions Prim-15 dorsal aorta membrane IFL
Prim-15 dorsal aorta ventral region IFL
Prim-15 vascular endothelium membrane IFL
Prim-15 to High-pec dorsal aorta hematopoietic stem cell IFL
Prim-15 to High-pec dorsal aorta membrane IFL
Prim-15 to High-pec dorsal aorta ventral region IFL
Prim-15 to High-pec vascular endothelium membrane IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tet2mk17/mk17 ; s896Tg ; tet3mk18/mk18 ; mu122Tg standard conditions Prim-15 - High-pec dorsal aorta nucleus broken, abnormal
tet2mk17/mk17 ; s896Tg ; tet3mk18/mk18 ; mu122Tg standard conditions Prim-15 - High-pec endothelial to hematopoietic transition decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.