ZFIN ID: ZDB-FIG-151016-3
Frese et al., 2015 - RNA splicing regulated by RBFOX1 is essential for cardiac function in zebrafish. Journal of Cell Science   128(16):3030-40 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 3

rbfox1 deficency does not influence heart morphology. (A,B) H&E-stained sagittal histological sections of MO-control and MO1-rbfox1 morphant hearts at 72hpf. Morphants display normal heart morphology with distinct endocardial and myocardial cell layers in the atrium (A) and ventricle (V), and a clear differentiation and demarcation of the atrium and ventricle by the atrio-ventricular ring (AV). OFT, outflow tract. (C,D) Atrial- and ventricle-specific myosin heavy chains are expressed normally, also suggesting that there is normal molecular chamber specification [green, antibody against atrial-specific myosin (S46); red, antibody against ventricular and atrial myosin (MF20)]. (E–H) Ultrastructural analysis of heart and skeletal muscle cells of MO-control- and MO1-rbfox1-injected embryos at 48hpf showing organized sarcomere units (SU) with thin and thick myofilaments in well-aligned bundles and discernible AI-, M- and Z-bands.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT control Long-pec atrium
IHC Long-pec cardiac ventricle
IHC WT + MO3-rbfox1 standard conditions Long-pec atrium
IHC Long-pec cardiac ventricle
Ab-S46 IHC WT control Long-pec atrium
IHC WT + MO3-rbfox1 standard conditions Long-pec atrium
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-rbfox1 standard conditions Long-pec atrium dilated, abnormal
Long-pec cardiac muscle cell organized, normal
Long-pec heart sarcomere organized, normal
Long-pec skeletal muscle cell organized, normal
Protruding-mouth atrium dilated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.