ZFIN ID: ZDB-FIG-151016-2
Frese et al., 2015 - RNA splicing regulated by RBFOX1 is essential for cardiac function in zebrafish. Journal of Cell Science   128(16):3030-40 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 2

Knockdown of rbfox1 leads to cardiomyopathy and heart failure. (A–C) Lateral view of MO-control- and MO1- and MO2-rbfox1-injected embryos. After injection of 3ng MO1-rbfox1, 85% (D) and MO2-rbfox1, 72% (E) of morphants embryos develop cardiomyopathy and heart failure. Results are mean±s.d. (n=3). (F) cDNA analysis of rbfox1 morphants after injection of MO2-rbfox1 shows skipping of exon 6 (198-bp product) compared to a product including exon 6 (259bp product) in control-treated embryos. Sanger sequencing reveals that exon 6 is completely excluded, predictably leading to a frame shift of the coding sequence and premature stop in exon 7. (H,I) Fractional shortening (FS) of the ventricular chamber of MO-control- and MO1-rbfox1-injected embryos measured at the indicated developmental stages. Fractional shortening is significantly reduced in rbfox1 morphants after 48hpf and further declines at 96hpf. Results are mean±s.d. (n=10).

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-rbfox1 standard conditions Long-pec brain edematous, abnormal
Long-pec cardiac ventricle decreased contractility, abnormal
Long-pec heart decreased functionality, abnormal
Protruding-mouth cardiac ventricle decreased contractility, abnormal
Day 4 blood circulation arrested, abnormal
Day 4 cardiac ventricle collapsed, abnormal
Day 4 cardiac ventricle decreased contractility, abnormal
WT + MO4-rbfox1 standard conditions Long-pec brain edematous, abnormal
Long-pec cardiac ventricle decreased contractility, abnormal
Long-pec heart decreased functionality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.