ZFIN ID: ZDB-FIG-151002-39
Riemer et al., 2015 - A functional Bucky ball-GFP transgene visualizes germ plasm in living zebrafish. Gene expression patterns : GEP   18(1-2):44-52 Full text @ Gene Expr. Patterns
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 1

Buc antibody specificity. Confocal images of stage IB oocytes. Buc (green) labels the Balbiani body in wild type oocytes (A) but not in mutants bucp106 (B) and bucp43 (C). By contrast, perinuclear Vasa (red) is not changed. Dashed line outlines the nucleus. Lateral views, animal to the top. Scale bar: 10 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
buc Ab2-buc WT standard conditions Adult oocyte stage I mitochondrial cloud IHC
Ab2-buc bucp106re/p106re standard conditions Adult Not Detected oocyte stage I mitochondrial cloud IHC
Ab2-buc bucp43bmtb/p43bmtb standard conditions Adult Not Detected oocyte stage I mitochondrial cloud IHC
ddx4 Ab1-ddx4 WT standard conditions Adult oocyte stage I perinuclear region of cytoplasm IHC
Ab1-ddx4 bucp106re/p106re standard conditions Adult oocyte stage I perinuclear region of cytoplasm IHC
Ab1-ddx4 bucp43bmtb/p43bmtb standard conditions Adult oocyte stage I perinuclear region of cytoplasm IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bucp106re/p106re standard conditions Adult oocyte stage I mitochondrial cloud buc expression absent, abnormal
bucp43bmtb/p43bmtb standard conditions Adult oocyte stage I mitochondrial cloud buc expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Gene expression patterns : GEP for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene expression patterns : GEP, 18(1-2), Riemer, S., Bontems, F., Krishnakumar, P., Gömann, J., Dosch, R., A functional Bucky ball-GFP transgene visualizes germ plasm in living zebrafish, 44-52, Copyright (2015) with permission from Elsevier. Full text @ Gene Expr. Patterns