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ZFIN ID: ZDB-FIG-151001-30
Percival et al., 2015 - Variations in sister chromatid cohesion dysfunction in esco2 mutant zebrafish reflects the phenotypic diversity of Roberts Syndrome. Disease models & mechanisms   8(8):941-55 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Condition:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 5

Depletion of esco2 results in genomic instability. (A) Stills taken from time-lapse imaging videos demonstrating the variety of genomic instability observed in 24-hpf esco2 mutant embryos. Micronuclei, anaphase bridges and failed cytokinesis were observed, as well as ‘without error’ divisions. Arrowhead points towards micronuclei. Arrow points to an anaphase bridge. Time stamps are in minutes. (B) Average frequency of above division fates in wild-type (11 divisions taken from three embryos) and esco2 mutant (43 divisions from four embryos) embryos based on time-lapse imaging. Error bars show mean±s.d. between embryos. All wild-type cells underwent a normal division; therefore, there is no s.d. or error bar to report. (C) Western blot of γ-H2AX in protein lysates from 24-hpf esco2+/+ (AB), esco2 sib (+/+ and +/m) and esco2m/m embryos. Irradiated (IR) embryos (100Gy at 24hpf and collected at 30hpf) serve as a positive control. Relative intensity was calculated using ImageJ; each sample was normalized to α-tubulin intensity, and then relative expression was calculated against esco2+/+ (relative normalized intensity=1). (D) Quantification of the number of chromatids within metaphase spreads (ne20 spreads per genotype) from pooled (10-12 embryos) esco2+/+ (AB controls) and esco2m/m embryos. (E) Frequency of micronuclei observed in interphase cells of the tail region of embryos injected with H2afva-eGFP; CAAX-mCherry mRNA. Percentage is based on total number of micronuclei observed over the number of nuclei observed in interphase cells (n=3 embryos/genotype, >75 cells per field, mean±s.d., *P<0.05).

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab6-h2afx WB AB standard conditions Prim-5 whole organism
WB AB radiation Prim-5 whole organism
WB WT standard conditions Prim-5 whole organism
WB esco2hi2865Tg/hi2865Tg standard conditions Prim-5 whole organism
Phenotype Details
Fish Conditions Stage Phenotype
esco2hi2865Tg/hi2865Tg standard conditions Prim-5 cell chromosome amount, abnormal
Prim-5 cell micronucleus increased amount, abnormal
Prim-5 chromosome segregation process quality, abnormal
Prim-5 mitotic chromosome decondensation premature, abnormal
Prim-5 mitotic cytokinesis decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.