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ZIRC
ZFIN ID: ZDB-FIG-150608-2
Hermkens et al., 2015 - Sox7 controls arterial specification in conjunction with hey2 and efnb2 function. Development (Cambridge, England)   142(9):1695-704 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Pec-fin
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Pec-fin

Fig. 2

sox7 mutants show an altered morphology of the LDA while displaying normal DA-PCV segregation in the trunk. (A) Rhodamine-dextran angiograms (injected into the PCV at the position of the arrow) of sox7hu5626 fli1a:eGFP siblings (upper panel) and mutants (lower panel) at 2dpf. Insets depict the rhodamine-dextran channel of the boxed area with higher magnification highlighting the lack of dye uptake in the DA of sox7hu5626 mutants. (B) Transverse sections of fli1a:eGFP-positive sox7hu5626 sibling and mutant embryos at 2dpf, stained with anti-GFP (green) and DAPI (blue). In sox7hu5626 mutants, the aortic morphology is disturbed at the position of LDA/DA fusion (arrows) while being unaffected more anteriorly and posteriorly to this position. For relative positions of sections, see supplementary material Fig. S3. (C) Dorsal view of sox7hu5626 kdrl:mCherry;flt4:mCitrine sibling and mutant embryos at 2.5dpf. Right panel kdrl:mCherry channel only. Shunt formation (arrow) occurs in sox7hu5626 mutants at the position of LDA fusion. (L) DA, (lateral) dorsal aorta; PCV, posterior cardinal vein; CCV, common cardinal vein.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Citrine hu7135Tg; s916Tg standard conditions Pec-fin common cardinal vein IFL
sox7hu5626/hu5626; hu7135Tg; s916Tg standard conditions Pec-fin common cardinal vein IFL
EGFP sox7hu5626/+; y1Tg standard conditions Long-pec dorsal aorta IHC
Long-pec vasculature IFL
Long-pec vasculature IHC
sox7hu5626/hu5626; y1Tg standard conditions Long-pec dorsal aorta IHC
Long-pec vasculature IHC
Long-pec vasculature IFL
mCherry hu7135Tg; s916Tg standard conditions Pec-fin dorsal aorta IFL
sox7hu5626/hu5626; hu7135Tg; s916Tg standard conditions Pec-fin dorsal aorta IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sox7hu5626/hu5626; hu7135Tg; s916Tg standard conditions Pec-fin dorsal aorta malformed, abnormal
sox7hu5626/hu5626; y1Tg standard conditions Long-pec dorsal aorta malformed, abnormal
Long-pec trunk blood circulation arrested, abnormal
Long-pec trunk blood circulation decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development