ZFIN ID: ZDB-FIG-150506-23
Sasore et al., 2014 - Deciphering Combinations of PI3K/AKT/mTOR Pathway Drugs Augmenting Anti-Angiogenic Efficacy In Vivo. PLoS One   9:e105280 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to Day 5

Fig. 1

PI3K/Akt/mTOR gene expression in developing Tg(fli1:EGFP) zebrafish.

(A) RT-PCR and (B) qPCR examined the mRNA levels of zebrafish pik3ca, pik3r1, mTOR and akt1 genes. Embryos were harvested at 6, 13, 18 or 24 hours post fertilization (hpf) and eyes at 48, 72 or 120 hours post fertilization (hpf). (A). Relative expression, normalised to 18S rRNA, of pik3ca, pik3r1, mTOR and akt1 mRNA levels in developing zebrafish embryos (black columns) and eyes (white columns) expressed relative to the 6 hpf stage. Results are expressed as mean ± S.D. (n = 3), *P<0.05, **P<0.01.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
akt1s1 WT standard conditions Shield whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec eye RTPCR
Protruding-mouth eye RTPCR
Day 5 eye RTPCR
mtor WT standard conditions Shield whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec eye RTPCR
Protruding-mouth eye RTPCR
Day 5 eye RTPCR
pik3ca WT standard conditions Shield whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec eye RTPCR
Protruding-mouth eye RTPCR
Day 5 eye RTPCR
pik3r1 WT standard conditions Shield whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec eye RTPCR
Protruding-mouth eye RTPCR
Day 5 eye RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One