header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-150407-7
Levitas-Djerbi et al., 2015 - The Hypothalamic Leptin-Neurotensin-Hypocretin Neuronal Networks in Zebrafish. The Journal of comparative neurology   523(5):831-48 Full text @ J. Comp. Neurol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Adult

Fig. 2 The spatial and temporal expression pattern of nts and ntsr throughout zebrafish development. Ventral (A–D) and lateral (E–H) views of a wholemount ISH experiment in larvae, using a probe for nts. A–H: 24 hpf, 2 dpf, 4 dpf, and 6 dpf larvae expressing nts in the PO and H. At 4 and 6 dpf, nts is additionally observed in the PT. I–L: nts mRNA ISH pattern in 100 µm transversal adult brain sections. nts is expressed in the PM, TPp, PTN, Hv, Hd, and Hc of adult zebrafish brains. M–T: Dorsal (M,O), lateral (N,P), and 100 µm transversal adult brain section (Q–T) views of an ISH experiment using an ntsr probe. ntsr is widely expressed throughout the brain, including areas of the telencephalon, diencephalon (TP and hypothalamus), and hindbrain. PO, preoptic region; H, hypothalamus; PM, magnocellular preoptic nucleus; TPp, periventricular nucleus of the posterior tuberculum; PTN, posterior tuberal nucleus; Hv, ventral zone of the periventricular hypothalamus; Hd, dorsal zone of the periventricular hypothalamus, Hc, caudal zone of the periventricular hypothalamus; TC, telencephalic cluster; Pr, pretectum; PT, posterior tuburculum; PG, pineal gland; h, hindbrain; Vs, supracommissural nucleus of V; Ppa, parvocellular preoptic nucleus, anterior part; PGZ, periventricular gray zone of optic tectum; PGN, preglomerular nucleus; LH, lateral hypothalamus; Val, lateral division of valvula cerebelli.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nts WT standard conditions Prim-5 hypothalamus ISH
Prim-5 preoptic area ISH
Long-pec hypothalamus ISH
Long-pec preoptic area ISH
Day 4 caudal periventricular hypothalamus ISH
Day 4 caudal tuberal nucleus ISH
Day 4 dorsal periventricular hypothalamus ISH
Day 4 magnocellular preoptic nucleus ISH
Day 4 periventricular nucleus ISH
Day 4 periventricular nucleus of caudal tuberculum ISH
Day 6 caudal periventricular hypothalamus ISH
Day 6 caudal tuberal nucleus ISH
Day 6 dorsal periventricular hypothalamus ISH
Day 6 magnocellular preoptic nucleus ISH
Day 6 periventricular nucleus ISH
Day 6 periventricular nucleus of caudal tuberculum ISH
Adult caudal periventricular hypothalamus ISH
Adult caudal tuberal nucleus ISH
Adult dorsal periventricular hypothalamus ISH
Adult magnocellular preoptic nucleus ISH
Adult periventricular nucleus ISH
Adult periventricular nucleus of caudal tuberculum ISH
ntsr1 WT standard conditions Prim-5 caudal tuberculum ISH
Prim-5 epiphysis ISH
Prim-5 hindbrain ISH
Prim-5 hypothalamus ISH
Prim-5 pretectum ISH
Prim-5 telencephalon ISH
Long-pec caudal tuberculum ISH
Long-pec epiphysis ISH
Long-pec hindbrain ISH
Long-pec hypothalamus ISH
Long-pec pretectum ISH
Long-pec telencephalon ISH
Adult caudal tuberculum ISH
Adult hypothalamus ISH
Adult telencephalon ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal The Journal of comparative neurology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Comp. Neurol.