ZFIN ID: ZDB-FIG-150401-17
Xu et al., 2014 - Maternal Vsx1 plays an essential role in regulating prechordal mesendoderm and forebrain formation in zebrafish. Developmental Biology   394(2):264-76 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Bud to 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Bud to 10-13 somites

Fig. 4

Maternal Vsx1 knockdown specifically inhibits forebrain formation. (A-F′) Expression patterns of six3b, eng2a and krox20 in uninjected wild-type and vsx1 tbMO injected embryos at 8-10 somite embryos. (G-H′) Comparison of relative expression levels and spatial patterns of forebrain marker rx3, mid-hindbrain boundary marker pax2a and hindbrain marker hoxb1b at bud stage. Brackets in G and H indicate the distance between forebrain region and mid-hindbrain boundary. Asterisks indicate the anterior end of forebrain. Black arrowheads indicate the location of mid-hindbrain boundary and gray arrowheads indicate the location of hindbrain. (A-H) Dorsal views with animal to the top. (A′-H′) Lateral views with animal to the top. The injected reagents are indicated at the left of each column. Riboprobes are indicated at the top of each group of figures.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b WT standard conditions 5-9 somites to 10-13 somites hindbrain ISH
WT + MO2-vsx1 standard conditions 5-9 somites to 10-13 somites hindbrain ISH
en2a WT standard conditions 5-9 somites to 10-13 somites midbrain ISH
WT + MO2-vsx1 standard conditions 5-9 somites to 10-13 somites midbrain ISH
hoxb1b WT standard conditions Bud hindbrain neural plate ISH
WT + MO2-vsx1 standard conditions Bud hindbrain neural plate ISH
pax2a WT standard conditions Bud midbrain hindbrain boundary neural plate ISH
WT + MO2-vsx1 standard conditions Bud midbrain hindbrain boundary neural plate ISH
rx3 WT standard conditions Bud forebrain neural plate ISH
WT + MO2-vsx1 standard conditions Bud forebrain neural plate ISH
six3b WT standard conditions 5-9 somites to 10-13 somites forebrain ISH
WT + MO2-vsx1 standard conditions 5-9 somites to 10-13 somites forebrain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-vsx1 standard conditions Bud forebrain development process quality, abnormal
5-9 somites - 10-13 somites forebrain development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 394(2), Xu, X., He, Y., Sun, L., Ma, S., Luo, C., Maternal Vsx1 plays an essential role in regulating prechordal mesendoderm and forebrain formation in zebrafish, 264-76, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.