Fig. 3

Maternal Vsx1 is essential for head formation and patterning in zebrafish. (A-H′′) Phenotype comparison of uninjected wild-type (A-B′′), vsx1 tbMO injected (C-D′′), vsx1 sbMO injected (E-F′′), vsx1 tbMO and ntl MO coinjected embryos at 24 hours post fertilization. The single images of embryos are presented in their lateral views with anterior to the top and dorsal to the right. The injected reagents are indicated at the left of each column. (B′, D′, F′, H′) and (B′′, D′′, F′′, H′′) are the magnified images of B, D, F and H at the head region and trunk region, respectively. Two arrowheads indicate the width of the notochord. Note that the vsx1 tbMO injected embryo is severely dorsalized, and the vsx1 tbMO and ntl MO coinjected embryo exhibits normal brain ventricles with no distinguishable notochord. (I) Proportion of different phenotypes in different experiments at 24 hpf. The injected reagents and their amounts are indicated at the bottom. Numbers of examined embryos are indicated at the top. BV: brain ventricle.