Fig. 3

Yap1 Regulates Endodermal Cell Survival

(A) Time-sequential (from eight-somite stage [8s] to 16-somite stage [16s]) images of Tg(sox17:GFP) embryos injected with MO indicated at the left. Arrows denote the fragmentation of sox17 promoter-driven GFP-positive cells. A set of representative images of eight independent experiments is shown. Dorsal view.

(B) Incidence of cardia bifia (black) and normal heart (white) in the morphants exhibiting less than 5 fragmented endodermal cells or more than 5 fragmented endodermal cells in the embryos treated with the MO indicated at the bottom.

(C) Bar graph shows the number of the TUNEL-positive cells among the GFP-positive endodermal cells of Tg(sox17:GFP) embryos at 14-somite stage (14s) injected with MO indicated at the bottom. ctrl MO, control morpholino. ^†p < 0.01 (versus ctrl morphants).

(D) Bar graph shows the number of the TUNEL-positive cells in a S1pr2 mutant (mil), heterozygous embryo (s1pr2^m93/+) and homozygous embryos (s1pr2^m93/m93). ^†p < 0.01.

(E) Gene expression profile analyzed by RNA-seq using RNAs obtained from the Tg(sox17:GFP) embryos uninjected (control) and injected with yap1 MO by sorting of GFP-positive endodermal cells. The graph shows the ratio of the reads per kilobase per million reads (RPKM) of the yap1 morphants-derived RNAs divided by that of the control. Among the genes we analyzed, those regulating cell survival are listed.

See also Figure S3 and Movies S1, S2, and S3.