Fig. 4

VPA decreases cell proliferation in the optic tectum of adult zebrafish. A: Timeline of drug treatment and method of quantification of cell number. B: Immunostaining with PCNA and BrdU in the dorsomedial area of the PGZ of the adult zebrafish optic tectum. Scale bar = 50 µm. C: The number of PCNA-positive cells (control: n = 3, VPA: n = 4). D: The number of BrdU-positive cells (control: n = 3; VPA: n = 4). E: The number of PCNA-positive cells in 10 caudal slices (control: n = 3; VPA: n = 4). F: The number of BrdU-positive cells in 10 caudal slices; 500 µM VPA treatment reduces the number of proliferating cells (control: n = 3; VPA: n = 4). G: Timeline of drug treatment. H: Immunostaining with HuC and BrdU in the PGZ of the adult zebrafish optic tectum. Scale bar = 100 µm. I: The number of BrdU-positive cells in 10 caudal slices (P = 0.26; control: n = 4; VPA: n = 3). J: The percentage of BrdU and HuC double-positive cells in BrdU-positive cells in 10 caudal slices. VPA administration does not relate to the promotion of neuronal differentiation (P = 0.32; control: n = 4; VPA: n = 3). CCe, corpus cerebelli; PGZ, periventricular gray zone; TeO, tectum opticum. **P < 0.01, *P < 0.05.