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ZFIN ID: ZDB-FIG-150326-38
Dozawa et al., 2014 - Valproic acid, a histone deacetylase inhibitor, regulates cell proliferation in the adult zebrafish optic tectum. Developmental dynamics : an official publication of the American Association of Anatomists   243(11):1401-15 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Condition:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 3

VPA administration does not relate to the promotion of neuronal differentiation. A: Timeline of drug treatment and BrdU labeling. B: Immunostaining with BrdU in the embryo zebrafish hindbrain with or without 2.0 mM VPA treatment. Scale bar = 100 µm, inset of 20 µm. C: Immunostaining with PCNA in the embryo zebrafish hindbrain with or without 2.0 mM VPA and 800 nM TSA treatment. Scale bar = 100 µm. D: Number of BrdU-positive cells with or without 2.0 mM VPA treatment (control: n = 5; VPA: n = 6). E: Number of PCNA-positive cells with or without 800 nM TSA treatment (P=0.12; control: n = 3; TSA: n = 3). F: Number of PCNA-positive cells with or without 2.0 mM VPA and 800 nM TSA treatment (control: n = 3; VPA+TSA: n = 3). G: Immunostaining with BrdU and HuC in the embryo zebrafish hindbrain with or without 2.0 mM VPA treatment. Scale bar = 100 µm, inset of 20 µm. H: The number of BrdU-positive cells (control: n = 3; VPA: n = 3). I: The percentage of BrdU and HuC double-positive cells in BrdU-positive cells (P=0.97; control: n = 3; VPA: n = 3). *P < 0.05.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-pcna IHC WT control Long-pec hindbrain
IHC WT chemical treatment: pharmaceutical Long-pec hindbrain
IHC WT chemical treatment: pharmaceutical Long-pec hindbrain
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: pharmaceutical Long-pec hindbrain cell population proliferation increased process quality, abnormal
WT chemical treatment: pharmaceutical Long-pec hindbrain cell population proliferation increased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.