ZFIN ID: ZDB-FIG-150326-33
Dyer et al., 2014 - Specification of sensory neurons occurs through diverse developmental programs functioning in the brain and spinal cord. Developmental dynamics : an official publication of the American Association of Anatomists   243(11):1429-39 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5

Fig. 3

Notch signalling components are expressed in the midbrain at early neurogenesis stages. Lateral views of the heads of 14-hpf embryos processed by in situ hybridisation (A–V). The Notch receptors elta, deltaB, deltaC, and deltaD (A–H), the Notch ligands notch1a, notch1b, notch2, and notch3 (I–P) and Notch-regulated genes her4, hey21, and hey4 (Q-V) were assessed for expression in the dorsal midbrain where MTN neurons will arise (bracket). No specific delta gene expression was detected, but notch1b and notch2 expression was observed in the dorsal midbrain. Both hey2 and her4 also showed expression in dorsal midbrain cells. Dorsal and orthogonal views of a 24-hpf Tg[elavl3:egfp] embryo processed by fluorescent in situ hybridisation and immunohistochemistry with anti-GFP reveals that expression of deltaB did not co-localise with EGFP in MTN neurons (intersect lines, W). Scale bars = 100 µm (A–W).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dla WT standard conditions 10-13 somites midbrain postero-ventral region ISH
10-13 somites telencephalon dorsal margin ISH
10-13 somites telencephalon ventral margin ISH
10-13 somites telencephalon ventral region ISH
10-13 somites thalamus ISH
dlb WT standard conditions 10-13 somites Not Detected midbrain ISH
10-13 somites telencephalon dorsal margin ISH
10-13 somites telencephalon ventral margin ISH
10-13 somites thalamus ISH
knu3Tg standard conditions Prim-5 midbrain ISH
dlc WT standard conditions 10-13 somites hindbrain ISH
10-13 somites telencephalon antero-dorsal region ISH
dld WT standard conditions 10-13 somites diencephalon ventral region ISH
10-13 somites telencephalon dorsal margin ISH
10-13 somites telencephalon ventral margin ISH
10-13 somites thalamus ISH
EGFP knu3Tg standard conditions Prim-5 mesencephalic nucleus of trigeminal nerve IHC
her4.1 WT standard conditions 10-13 somites diencephalon ventral region ISH
10-13 somites midbrain posterior region ISH
10-13 somites telencephalon dorsal margin ISH
10-13 somites telencephalon ventral margin ISH
10-13 somites thalamus ISH
hey1 WT standard conditions 10-13 somites brain ISH
hey2 WT standard conditions 10-13 somites brain ISH
10-13 somites Not Detected midbrain hindbrain boundary neural rod ISH
notch1a WT standard conditions 10-13 somites brain ISH
notch1b WT standard conditions 10-13 somites brain ISH
notch2 WT standard conditions 10-13 somites brain ISH
notch3 WT standard conditions 10-13 somites brain ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.