Fig. 2

The c3 intron-less reporter recapitulates endogenous etv2 expression pattern during early but not late segmentation stages. Two-color fluorescent ISH of c3 transgenic embryos at 6-8 som in the ALPM (A-C) and at 20-22 som in the cardiac disc (D-F) for the detection of endogenous transcripts of etv2 in red (A and D) and concomitant detection of kaede transcripts from the c3 transgene in green (B and E), merged in (C and F). At 6-8 som, maximal overlap of both signals was observed (C), however at 20-22 som, cardiac c3 transgene expression did not overlap with endogenous etv2 (F) whereas the c2 egfp transgene recapitulated etv2 expression at that stage (G and H). Embryos are dorsal with anterior to the top. Images shown are snapshots from 3D views with bars ≈100 µm related to the background grid (1 unit=45.18 µm in A-C, 22.59 µm in D-F, and 22.54 µm in G-I).