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ZFIN ID: ZDB-FIG-150323-2
Sawamiphak et al., 2014 - Interferon Gamma Signaling Positively Regulates Hematopoietic Stem Cell Emergence. Developmental Cell   31:640-653 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec

Fig. 2

Cell Proliferation and Survival in the Axial Vessels Do Not Appear to Be Affected by Gain or Loss of Function of Ifng1-2 Signaling

(A–C) ifng1-2 overexpression increases HSC number without affecting cell division. HSCs (itga2b:EGFP+kdrl:HRAS-mCherry+, green+red+) that exit the G0 phase, labeled by PCNA immunostaining (blue), in control embryos not harboring the Tg(hsp:ifng1-2-V5) transgene (A), and Tg(hsp:ifng1-2-V5) embryos (B) are indicated by white circles. PCNA HSCs are marked with white arrowheads. (C) Percentage of PCNA+HSCs per total HSCs in the dorsal aorta. Values represent means ± SEM, n = 9 embryos per group, n.s., not significant (p > 0.05).

(D–M) Ifng1-2 and Crfb17 knockdown has no effect on apoptosis. Apoptotic cells (green, white arrowheads) visualized by Tg(Tbp:GAL4;UAS:secA5-YFP) expression (D–I) and TUNEL assay (J–M) in the axial vessels of MOCK-injected (D, G, and J), crfb17 MO-injected (E and L), and Ifng1-2 MO-injected (H and K) embryos at 52–54 hpf. Numbers of Tbp:GAL4;UAS:secA5-YFP+ cells (F and I) and TUNEL+kdrl:HRAS-mCherry+ cells per 500 µm dorsal aorta length (M) are shown as means ± SEM, n = 11–25 embryos, n.s. p > 0.05. All images are lateral views, dorsal up and anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP la2Tg; s896Tg control Long-pec hematopoietic stem cell IHC
la2Tg; s896Tg; s994Tg heat shock Long-pec hematopoietic stem cell IHC
mCherry la2Tg; s896Tg control Long-pec hematopoietic stem cell IHC
la2Tg; s896Tg; s994Tg heat shock Long-pec hematopoietic stem cell IHC
s896Tg control Long-pec vasculature IHC
Long-pec ventral wall of dorsal aorta hematopoietic stem cell IHC
s896Tg + MO1-ifng1 standard conditions Long-pec vasculature IHC
Long-pec ventral wall of dorsal aorta hematopoietic stem cell IHC
s896Tg + MO2-ifngr1 standard conditions Long-pec vasculature IHC
Long-pec ventral wall of dorsal aorta hematopoietic stem cell IHC
YFP f12Tg; f13Tg control Long-pec vasculature IFL
f12Tg; f13Tg + MO1-ifng1 standard conditions Long-pec vasculature IFL
f12Tg; f13Tg + MO2-ifngr1 standard conditions Long-pec vasculature IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-pcna IHC la2Tg; s896Tg control Long-pec ventral wall of dorsal aorta hematopoietic stem cell
IHC la2Tg; s896Tg; s994Tg heat shock Long-pec ventral wall of dorsal aorta hematopoietic stem cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 31, Sawamiphak, S., Kontarakis, Z., Stainier, D.Y., Interferon Gamma Signaling Positively Regulates Hematopoietic Stem Cell Emergence, 640-653, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell