FIGURE

Fig. 8

ID
ZDB-FIG-150318-13
Publication
Gerlach et al., 2014 - Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta
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Fig. 8

Ectopic expression of podocyte genes and early developmental transcription factors in the pronephros is not a general consequence of embryonic defects in fluid flow. WISH was used to assess gene expression using antisense riboprobes to detect wt1a, pax2a, or podxl (purple) in (A) wild-types, (B) prkCI morphants, (C) ift88 morphants, (D) tnnt2a morphants, (E) double ift88/tnnt2a morphants, and (F) mpp5a morphants. (A–E) Knockdown of prkCI was the only circumstance that led to high levels of ectopic expression of podocyte genes in the pronephros tubule. (F) (Top row) Most mpp5a morphants displayed no ectopic pronephros expression, but (bottom row) a subset (<10%) displayed low levels of ectopic wt1a, pax2a, or podxl in the tubule and also diffuse ectopic expression.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.