ZFIN ID: ZDB-FIG-141216-12
Maragh et al., 2014 - Rbm24a and Rbm24b Are Required for Normal Somitogenesis. PLoS One   9:e105460 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5

Fig. 3

Notch-signaling pathway transcripts are depleted in somites of rbm24aMO and rbm24bMO embryos.

ISH images of the PSM of dorsally oriented 13-15 somite and laterally oriented 24 hpf ctrlMO, rbm24aMO and rbm24bMO embryos. 13-15 somite embryos are shown in 2 different temporal stages of development. Spatial expression of dlc (A-F), dld (G-L), her1 (M-R) and her7 (S-X) and tbx16 (Y-DD). Asterisks highlight cycling expression. n = 8-12 embryos stained per time-point with no more than 1 deviating from the displayed expression. arrow, regions of interest for PSM expression.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlc WT control 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
Prim-5 segmental plate ISH
WT + MO1-rbm24a standard conditions 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
Prim-5 segmental plate ISH
WT + MO1-rbm24b standard conditions 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
Prim-5 segmental plate ISH
dld WT control 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
Prim-5 segmental plate ISH
WT + MO1-rbm24a standard conditions 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
Prim-5 segmental plate ISH
WT + MO1-rbm24b standard conditions 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
Prim-5 segmental plate ISH
her1 WT control 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
Prim-5 segmental plate ISH
WT + MO1-rbm24a standard conditions 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
Prim-5 segmental plate ISH
WT + MO1-rbm24b standard conditions 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
Prim-5 segmental plate ISH
her7 WT control 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
Prim-5 segmental plate ISH
WT + MO1-rbm24a standard conditions 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
Prim-5 segmental plate ISH
WT + MO1-rbm24b standard conditions 10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
Prim-5 segmental plate ISH
tbx16 WT control 10-13 somites to 14-19 somites segmental plate ISH
Prim-5 segmental plate ISH
WT + MO1-rbm24a standard conditions 10-13 somites to 14-19 somites segmental plate ISH
Prim-5 segmental plate ISH
WT + MO1-rbm24b standard conditions 10-13 somites to 14-19 somites segmental plate ISH
Prim-5 segmental plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One