ZFIN ID: ZDB-FIG-141001-9
Shi et al., 2014 - Acetylcholine serves as a derepressor in Loperamide-induced Opioid-Induced Bowel Dysfunction (OIBD) in zebrafish. Scientific Reports   4:5602 Full text @ Sci. Rep.
Observed In:
Stage: Day 6

Fig. 6

(a) The larval fish do not show an obvious developmental defect when treated by ACh-Cl and LH+ACh-Cl for 12 hours compared with control. Left panels are in the BF channel, whereas right are in the GFP channel. (b–c) Quantification data indicate that the effect of exogenous ACh-Cl on gut peristalsis at 4 dpf (b) and 6 dpf (c) after transit incubation for 10–30 minutes. 10, 100, 1000, 2500 and 5000 mg/L ACh-Cl had no influence on the gut movement frequency at 4 dpf (b), whereas 2500 mg/L promote the movement ability obviously at 6 dpf (c). (d) Quantification data show that treatment of exogenous ACh-Cl for 10–30 minutes partially rescue the inhibition phenotype of gut peristalsis caused by LH. (e) Quantification data show that acetylcholinesterase (AChE) inhibits the recovered phenotype by exogenous ACh-Cl. P value is statistical with corresponding control.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: acetylcholine chloride Day 6 gut peristalsis increased frequency, abnormal
WT chemical treatment: loperamide hydrochloride Day 6 gut peristalsis decreased frequency, abnormal
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