ZFIN ID: ZDB-FIG-141001-9
Shi et al., 2014 - Acetylcholine serves as a derepressor in Loperamide-induced Opioid-Induced Bowel Dysfunction (OIBD) in zebrafish. Scientific Reports   4:5602 Full text @ Sci. Rep.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 6

Fig. 6

(a) The larval fish do not show an obvious developmental defect when treated by ACh-Cl and LH+ACh-Cl for 12 hours compared with control. Left panels are in the BF channel, whereas right are in the GFP channel. (b–c) Quantification data indicate that the effect of exogenous ACh-Cl on gut peristalsis at 4 dpf (b) and 6 dpf (c) after transit incubation for 10–30 minutes. 10, 100, 1000, 2500 and 5000 mg/L ACh-Cl had no influence on the gut movement frequency at 4 dpf (b), whereas 2500 mg/L promote the movement ability obviously at 6 dpf (c). (d) Quantification data show that treatment of exogenous ACh-Cl for 10–30 minutes partially rescue the inhibition phenotype of gut peristalsis caused by LH. (e) Quantification data show that acetylcholinesterase (AChE) inhibits the recovered phenotype by exogenous ACh-Cl. P value is statistical with corresponding control.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: acetylcholine chloride Day 6 gut peristalsis increased frequency, abnormal
WT chemical treatment: loperamide hydrochloride Day 6 gut peristalsis decreased frequency, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.