ZFIN ID: ZDB-FIG-141001-8
Shi et al., 2014 - Acetylcholine serves as a derepressor in Loperamide-induced Opioid-Induced Bowel Dysfunction (OIBD) in zebrafish. Scientific Reports   4:5602 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Condition:
Anatomical Term:
Stage: Day 6
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 6

Fig. 5

(a) Immunohistochemical staining of HuC/D in both control and LH-treated fish at 6 dpf. The data show no significant difference between the control and chemical application groups. Left panels show the whole body staining pattern of HuC/D, whereas middle and right panels represent high magnifications of the areas that are boxed in left. Right panels are the images of middle merged with DIC. (b) The AChE activity detection assay reveal that AChE activity is significantly reduced after LH treatment (right panels) compared with control (left panels) in the gut but not elsewhere in the body. Upper panels show the whole body staining pattern of Ache, whereas bottom panels represent high magnifications of the boxed images. The red arrows in b indicate the intestinal bulb region where peristalsis is identified.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC WT control Day 6 enteric neuron
IHC WT chemical treatment: loperamide hydrochloride Day 6 enteric neuron
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: loperamide hydrochloride Day 6 gut acetylcholinesterase activity decreased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.