ZFIN ID: ZDB-FIG-141001-5
Shi et al., 2014 - Acetylcholine serves as a derepressor in Loperamide-induced Opioid-Induced Bowel Dysfunction (OIBD) in zebrafish. Scientific Reports   4:5602 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: Long-pec to Day 6

Fig. 2

(a) The staining patterns of almarBlue reveal the gut lumen (white arrowheads) and circulating blood cells (white arrows) at 2–6 dpf in the lateral view. (b) Green signals are universally detected in Tg(actb2:HyPer)pku326 before 3 dpf, and the signals enhance in the intestinal epithelial cells at 6 dpf (white arrows). (c) RT-PCR reveal the efficient block of duox transcript splicing via MO mediated genetic knockdown. (d) The signals of the ROS/redox probes reduce, but not exclusively disappear, in the intestinal tract after duox is genetic knockdown by MO. White arrowheads indicate the signals in the intestinal tract.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
HyPer pku326Tg standard conditions Long-pec Not Detected intestinal epithelial cell IFL
Protruding-mouth Not Detected intestinal epithelial cell IFL
Day 6 intestinal epithelial cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.