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ZIRC
ZFIN ID: ZDB-FIG-140819-6
Trevarrow et al., 1990 - Organization of hindbrain segments in the zebrafish embryo. Neuron   4:669-679 Full text @ Neuron
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 4

Fig. 5 Rows of Radial Glial Fibers Separate the Segment Centers from Their Borders

(A) The zrf-1 antibody labels typically shaped radial glial fibers that extend from the cells at the central canal to the pial surface in this transverse section of a 4 day zebrafish spinal cord. not: notochord. The spinal cord and hindbrain were labeled similarly by the other rf antibodies and by a mouse monoclonal antibody (from ICN Immunobiological) to GFAP (data not shown).

(B) In the hindbrain, bundles of dorso-ventrally running glial fibers (asterisks) are present between the segment borders (large arrows) and centers. This sagittal section, at 48 hr, was labeled with a mixture of zrf-1, zrf-2, and zrf-4 antibodies to stain the glial fibers heavily and with zn-5 to label commissural axons (arrowheads). The axon bundles run orthogonally to the glial fiber bundles, and the two appear to be in close contact.

(C) The glial bundles, cut across in this horizontal sec8tion and labeled with zrf-1 at 48 hr, are arranged in parallel transverse rows (arrowheads), two in each segment. Retrogradely labeled reticulospinal neurons are dorkly stained in the Mi1 and Mi2 segments and mark the locations of he segment centers. The hindbrain-midbrain boundary is to the left.

Scale bars: 25 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zrf-1 IHC WT standard conditions Long-pec hindbrain glial cell
IHC Day 4 radial glial cell
zrf-2 IHC WT standard conditions Long-pec hindbrain glial cell
zrf-4 IHC WT standard conditions Long-pec hindbrain glial cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neuron for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neuron