ZFIN ID: ZDB-FIG-140818-17
Bae et al., 2003 - A homeobox gene, pnx, is involved in the formation of posterior neurons in zebrafish. Development (Cambridge, England)   130(9):1853-1865 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 1-4 somites

Fig. 7 Pnx can promote primary neurogenesis independently of Delta-Notch signaling. (A-H) Ten picograms of pnx and β-galactosidase RNA, 20 pg of VP16pnx and β-galactosidase RNA, or 2 ng of pnx MO (recognizing the translational initiation site) were injected into embryos of crossed mib mutants, fixed at the three-somite stage, and stained with ngn1, elavl3 and islet2 (A-H). The blue region stained with X-gal contained the injected RNA (A-D). The mib homozygous mutant embryos were detected by an increase in Rohon-Beard neurons. Misexpression of pnx in mib expanded the ngn1- (A) and elavl3-expressing stripes (B) but did not change the density of those cells within the stripes. Expression of VP16-Pnx (C,D) or injection of pnx MO (E,F) into the mib mutant embryos reduced the numbers of ngn1 (C,E,F) and elavl3-expressing cells (D) at the three-somite stage. islet2-expressing primary motoneurons were reduced in the pnx MO-injected mib mutant embryos (H). (I-N) One hundred picograms of XDlstu RNA and β-galactosidase RNA were co-injected with 10 pg of pnx RNA or 20 pg VP16pnx RNA into the wild-type embryos, and the expression of ngn1 and elavl3 at the three-somite stage was detected. Expression of XDlstu increased the density of ngn1- (I) and elavl3-expressing cells (J), and co-expression of Pnx expanded the ngn1- (K) and elavl3-expressing (L) stripes without affecting the densities of ngn1- and elavl3-positive cells. Co-expression of VP16-Pnx reduced the numbers of ngn1- (M) and elavl3-expressing cells (N). (A-F,I-N) Dorsal views. (G,H) Lateral views in the spinal cord region. (O) Model for the role of pnx in posterior neurogenesis.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
isl2a mib1m178/m178 standard conditions 1-4 somites primary motor neuron ISH
mib1m178/m178 + MO2-pnx standard conditions 1-4 somites primary motor neuron ISH
neurog1 mib1m178/m178 standard conditions 1-4 somites neuroectoderm ISH
mib1m178/m178 + MO2-pnx standard conditions 1-4 somites neuroectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mib1m178/m178 standard conditions 1-4 somites Rohon-Beard neuron increased amount, abnormal
mib1m178/m178 + MO2-pnx standard conditions 1-4 somites primary motor neuron decreased amount, abnormal
Acknowledgments:
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