ZFIN ID: ZDB-FIG-140812-20
Wiweger et al., 2014 - Possible effects of EXT2 on mesenchymal differentiation--lessons from the zebrafish. Orphanet journal of rare diseases   9:35 Full text @ Orphanet J Rare Dis
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 3

Homozygous ext2 mutant displays musco-skeletal phenotype. Whole mount immunolocalisation at 4dpf using MF-20 antibody for muscles (green) and collagen II for cartilage (red) shows thicker and shorter muscles fitting the malformed cartilaginous skeleton in the ext2-/- fish. Muscles: intermandibularis anterior (ima), intermandibularis posterior (imp), adductor mandibulae (am), interhyoideus (ih), hyohyoideus (hh), levator arcus palatine (lap), adductor hyoideus (ah), dilator operculi (do), sternohyoideus (sh) and adductor operculi (ao). Note missing hh muscle and, marked with a star, increased musculature around heart (H) in ext2-/-. Scale = 0.1 mm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT standard conditions Day 4 adductor hyohyoid
IHC Day 4 adductor mandibulae
IHC Day 4 adductor operculi
IHC Day 4 dilatator operculi
IHC Day 4 hyohyoideus
IHC Day 4 interhyoideus
IHC Day 4 levator arcus palatini
IHC Day 4 sternohyoid
IHC Day 4 ventral intermandibularis anterior
IHC Day 4 ventral intermandibularis posterior
IHC ext2to273b/to273b standard conditions Day 4 adductor hyohyoid
IHC Day 4 adductor mandibulae
IHC Day 4 adductor operculi
IHC Day 4 dilatator operculi
IHC Day 4 interhyoideus
IHC Day 4 levator arcus palatini
IHC Day 4 sternohyoid
IHC Day 4 ventral intermandibularis anterior
IHC Day 4 ventral intermandibularis posterior
Phenotype Details
Fish Conditions Stage Phenotype
ext2to273b/to273b standard conditions Day 4 adductor hyohyoid increased width, abnormal
Day 4 adductor hyohyoid shortened, abnormal
Day 4 adductor mandibulae increased width, abnormal
Day 4 adductor mandibulae shortened, abnormal
Day 4 adductor operculi increased width, abnormal
Day 4 adductor operculi shortened, abnormal
Day 4 cranial cartilage shape, abnormal
Day 4 dilatator operculi increased width, abnormal
Day 4 dilatator operculi shortened, abnormal
Day 4 head muscle increased width, abnormal
Day 4 head muscle shortened, abnormal
Day 4 heart anatomical region has extra parts of type muscle, abnormal
Day 4 hyohyoideus absent, abnormal
Day 4 interhyoideus increased width, abnormal
Day 4 interhyoideus shortened, abnormal
Day 4 levator arcus palatini increased width, abnormal
Day 4 levator arcus palatini shortened, abnormal
Day 4 sternohyoid increased width, abnormal
Day 4 sternohyoid shortened, abnormal
Day 4 ventral intermandibularis anterior increased width, abnormal
Day 4 ventral intermandibularis anterior shortened, abnormal
Day 4 ventral intermandibularis posterior increased width, abnormal
Day 4 ventral intermandibularis posterior shortened, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Orphanet journal of rare diseases for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Orphanet J Rare Dis