ZFIN ID: ZDB-FIG-140527-35
Rydeen et al., 2014 - Cyp26 enzymes are required to balance the cardiac and vascular lineages within the anterior lateral plate mesoderm. Development (Cambridge, England)   141:1638-48 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 5-9 somites

Fig. S1

Cyp26a1 and cyp26c1 act redundantly in hindbrain patterning. (A-F) Flatmounted double ISH with eng2a (purple) and egr2b (red) at 8s. Singular cyp26a1 and cyp26c1 deficient and the gir mutants do not have significant changes in hindbrain patterning. (E,F) Cyp26 deficient and gir+C embryos have significant anterior shifts in the posterior rhombomeres, resulting in a loss of the rhombomeres three and four. Equivalent results were demonstrated in (Hernandez et al., 2007). All images are dorsal views with anterior left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b WT standard conditions 5-9 somites hindbrain neural rod ISH
WT + MO1-cyp26c1 standard conditions 5-9 somites hindbrain neural rod ISH
WT + MO1-cyp26c1 + MO4-cyp26a1 + MO5-cyp26a1 standard conditions 5-9 somites hindbrain neural rod ISH
WT + MO4-cyp26a1 + MO5-cyp26a1 standard conditions 5-9 somites hindbrain neural rod ISH
cyp26a1rw716/rw716 standard conditions 5-9 somites hindbrain neural rod ISH
cyp26a1rw716/rw716 + MO1-cyp26c1 standard conditions 5-9 somites hindbrain neural rod ISH
en2a WT standard conditions 5-9 somites hindbrain neural rod ISH
WT + MO1-cyp26c1 standard conditions 5-9 somites hindbrain neural rod ISH
WT + MO1-cyp26c1 + MO4-cyp26a1 + MO5-cyp26a1 standard conditions 5-9 somites hindbrain neural rod ISH
WT + MO4-cyp26a1 + MO5-cyp26a1 standard conditions 5-9 somites hindbrain neural rod ISH
cyp26a1rw716/rw716 standard conditions 5-9 somites hindbrain neural rod ISH
cyp26a1rw716/rw716 + MO1-cyp26c1 standard conditions 5-9 somites hindbrain neural rod ISH
hoxb4a WT standard conditions 5-9 somites hindbrain neural rod ISH
WT + MO1-cyp26c1 standard conditions 5-9 somites hindbrain neural rod ISH
WT + MO1-cyp26c1 + MO4-cyp26a1 + MO5-cyp26a1 standard conditions 5-9 somites hindbrain neural rod ISH
WT + MO4-cyp26a1 + MO5-cyp26a1 standard conditions 5-9 somites hindbrain neural rod ISH
cyp26a1rw716/rw716 standard conditions 5-9 somites hindbrain neural rod ISH
cyp26a1rw716/rw716 + MO1-cyp26c1 standard conditions 5-9 somites hindbrain neural rod ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development