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ZIRC
ZFIN ID: ZDB-FIG-140527-24
Rydeen et al., 2014 - Cyp26 enzymes are required to balance the cardiac and vascular lineages within the anterior lateral plate mesoderm. Development (Cambridge, England)   141:1638-48 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-25

Fig. 2

Cyp26-deficient embryos have increased atrial differentiation and cell number. (A-L) Cardiac differentiation marker expression in wild-type, cyp26a1-deficient, cyp26c1-deficient and cyp26-deficient embryos. Singular knock down of cyp26a1 (D-F) or cyp26c1 (G-I) does not affect cardiac differentiation. Double knockdown (J-L) shows an expansion of amhc expression. (M) Area measurements indicate that amhc expression is expanded in Cyp26-deficient embryos. For myl7: WT, n=38; Cyp26a1-deficient, n=12; Cyp26c1-deficient, n=18; Cyp26-deficient, n=30. For vmhc: WT, n=40; Cyp26a1-deficient, n=19; Cyp26c1-deficient, n=16; Cyp26-deficient, n=37. For amhc: WT, n=33; Cyp26a1-deficient, n=17; Cyp26c1-deficient, n=17; Cyp26-deficient, n=41. (N) RT-qPCR indicates that only amhc expression is increased in Cyp26-deficient embryos. (O) Cardiomyocyte counts reveal a specific increase in atrial cells in Cyp26-deficient embryos at 36hpf. WT, n=53; Cyp26a1-deficient, n=15; Cyp26c1-deficient, n=15; Cyp26 deficient, n=51. All images are dorsal views with anterior upwards. Significant differences compared with controls are indicated (*P<0.05). Error bars indicate s.d.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myh6 WT standard conditions 20-25 somites heart primordium ISH
WT + MO1-cyp26c1 standard conditions 20-25 somites heart primordium ISH
WT + MO1-cyp26c1 + MO4-cyp26a1 + MO5-cyp26a1 standard conditions 20-25 somites heart primordium ISH
WT + MO4-cyp26a1 + MO5-cyp26a1 standard conditions 20-25 somites heart primordium ISH
myh7 WT standard conditions 20-25 somites heart primordium ISH
WT + MO1-cyp26c1 standard conditions 20-25 somites anterior lateral plate mesoderm ISH
20-25 somites anterior lateral plate mesoderm cardiovascular system ISH
20-25 somites cardiovascular system ISH
20-25 somites heart primordium ISH
WT + MO1-cyp26c1 + MO4-cyp26a1 + MO5-cyp26a1 standard conditions 20-25 somites heart primordium ISH
WT + MO4-cyp26a1 + MO5-cyp26a1 standard conditions 20-25 somites heart primordium ISH
myl7 WT standard conditions 20-25 somites heart primordium ISH
WT + MO1-cyp26c1 standard conditions 20-25 somites heart primordium ISH
WT + MO1-cyp26c1 + MO4-cyp26a1 + MO5-cyp26a1 standard conditions 20-25 somites heart primordium ISH
WT + MO4-cyp26a1 + MO5-cyp26a1 standard conditions 20-25 somites heart primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cyp26c1 + MO4-cyp26a1 + MO5-cyp26a1 standard conditions Prim-25 atrium has fewer parts of type cardiac muscle cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development