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ZIRC
ZFIN ID: ZDB-FIG-140523-54
Chen et al., 2014 - The development of zebrafish tendon and ligament progenitors. Development (Cambridge, England)   141:2035-45 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 4

Fig. 2 Tendon genes mark discrete domains joining muscle and cartilage. (A,B) scxa is expressed at points of attachment of muscle-to-cartilage and cartilage-to-cartilage in the craniofacial tissue at 60hpf. Images were generated by overlaying the bright-field and fluorescent channels. (C) scxa and tnmd are co-expressed at the base of the cleithrum (arrow). (D-F) scxa and xirp2a are co-expressed in muscle attachment points, e.g. where the interhyoideus and intermandibularis muscles intersect (D, arrowhead; enlarged in E). Single channels of confocal image are shown in E. (G-I) Colocalization of scxa and tnmd is detected between 60 and 80hpf. Expression of scxa and tnmd transcripts is temporally dynamic: there is robust expression of scxa at 60hpf followed by weaker expression after 80hpf; tnmd is weakly expressed at 60hpf and increases in expression after 80hpf. (J) At 96hpf, xirp2a and tnmd are co-expressed in regions proximal to the muscle (arrowhead). (K-M) scxa and xirp2a are co-expressed in the tail myosepta in regions medial (L) and lateral (M) to the notochord. Single channels of confocal images are shown in L,M and an asterisk marks the corresponding myoseptum in the same embryo. (N,O) At 96hpf, tnmd and col1a2 are co-expressed in regions medial to the palatoquadrate (N, arrow), at the sternohyoideus connection point (N, arrowhead), and in the myosepta (O). Ventral (A,D-J,N) and lateral (B,C,K-M,O) views of flat-mounted embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col1a2 WT standard conditions Day 4 myoseptum ISH
Day 4 pharyngeal arch portion of connective tissue ISH
scxa WT standard conditions Long-pec myoseptum ISH
Pec-fin cleithrum basal region ISH
Pec-fin pharyngeal arch portion of connective tissue ISH
Pec-fin pharyngeal arch tendon ISH
Protruding-mouth pharyngeal arch tendon ISH
tnmd WT standard conditions Pec-fin cleithrum basal region ISH
Pec-fin pharyngeal arch portion of connective tissue ISH
Protruding-mouth pharyngeal arch portion of connective tissue ISH
Day 4 myoseptum ISH
Day 4 pharyngeal arch portion of connective tissue ISH
xirp2a WT standard conditions Long-pec myoseptum ISH
Pec-fin pharyngeal arch portion of connective tissue ISH
Protruding-mouth pharyngeal arch portion of connective tissue ISH
Protruding-mouth pharyngeal arch tendon ISH
Day 4 pharyngeal arch portion of connective tissue ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-A4.1025 IHC WT standard conditions Pec-fin muscle
IHC Pec-fin pharyngeal musculature
IHC Protruding-mouth pharyngeal musculature
Ab1-col2a IHC WT standard conditions Pec-fin pharyngeal arch cartilage
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development