ZFIN ID: ZDB-FIG-140421-10
Zigman et al., 2014 - Hoxb1b controls oriented cell division, cell shape and microtubule dynamics in neural tube morphogenesis. Development (Cambridge, England)   141(3):639-649 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to 20-25 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1-4 somites to 20-25 somites

Fig. 2

Normal cell polarity but disrupted convergence in the hoxb1b-/- neural keel. (A) Normal neuroepithelial polarity at the single-cell level in hoxb1b-/- based on sub-apical Pard3-GFP (black) at 21 hpf, apical centrosomes (γ-tubulin, red) and apically enriched F-actin (green), all analyzed at 19 hpf. Neuronal differentiation occurs at wild-type levels but generates HuC/D-positive neurons (yellow) in abnormal medial postitions in hoxb1b-/- at 20 hpf. (B) Dynamics of neural keel convergence in time-lapses of wild-type and hoxb1b-/- transgenic Gt(Ctnna-Citrine)ct3a embryos with multiphoton imaging. Upper panels show a single z-section in dorsal view one-third of the way through the time lapse, corresponding to about the 10-somite stage. Lower panels: kymograph of the position marked by a yellow dotted line, beginning at the 3-somite stage (11 hpf) and continuing until the 18 somite stage (18 hpf). A single lumen appears at the midline in wild type (arrowhead) whereas a duplicated midline appears at about the same stage in hoxb1b-/-. The width of the neural keel is depicted as red brackets. (B2) Quantification of neural keel width measured at three positions in r3/4 over the period in the kymograph. Convergence is significantly slowed in hoxb1b-/- compared with wild-type siblings (n=2 embryos for each genotype). Data are mean ± s.e.m. (C) Assessment of planar cell polarity by GFP-Prickle (green) localization at 18 hpf. Tg(egr2b:KalTA4) transgene (purple) is included to identify rhombomere 3. Mosaically expressed GFP-Pk localizes in puncta, which in wild type lie on anterior progenitor cell membranes (white arrows). In hoxb1b-/-, GFP-Pk localizes to puncta that lie on one side of mis-oriented progenitor cells. Lower panels show GFP-Pk signal presented in pseudocolors. (C2) Quantitative analysis of GFP-Prickle subcellular localization. (D) Schematic of cell polarity in hoxb1b-/-. The anterior-posterior animal axes are marked by double arrows in all panels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Citrine ctnna1ct3aGt standard conditions 1-4 somites to 14-19 somites hindbrain adherens junction IFL
10-13 somites hindbrain adherens junction IFL
hoxb1bb1219/b1219; ctnna1ct3aGt standard conditions 1-4 somites to 14-19 somites hindbrain adherens junction IFL
10-13 somites hindbrain adherens junction IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tubg1 IHC WT standard conditions 20-25 somites hindbrain centrosome
IHC hoxb1bb1219/b1219 standard conditions 20-25 somites hindbrain centrosome
Ab2-tubg1 IHC WT standard conditions 20-25 somites hindbrain centrosome
IHC hoxb1bb1219/b1219 standard conditions 20-25 somites hindbrain centrosome
Phenotype Details
Fish Conditions Stage Phenotype
ctnna1ct3aGt standard conditions 1-4 somites - 14-19 somites fourth ventricle development process quality, abnormal
1-4 somites - 14-19 somites hindbrain neural keel formation process quality, abnormal
1-4 somites - 14-19 somites rhombomere 3 neural keel formation process quality, abnormal
1-4 somites - 14-19 somites rhombomere 4 neural keel formation process quality, abnormal
hoxb1bb1219/b1219 standard conditions 20-25 somites establishment or maintenance of cell polarity process quality, normal
20-25 somites hindbrain cell disoriented, abnormal
20-25 somites hindbrain neuron mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development