FIGURE

Fig. 1

ID
ZDB-FIG-140421-1
Publication
Wilkins et al., 2014 - A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

krt18 and Tg(krt18:egfp) are expressed in the developing biliary system. (A) Whole-mount in situ hybridization of 5 dpf wild-type larva (left lateral view), showing gallbladder expression of krt18 (arrow). (B) Combined immunostaining and fluorescent in situ hybridization of 5 dpf Tg(tp1bglob:egfp) larva (left lateral view), showing Notch-responsive GFP protein in intrahepatic bile ducts within the liver (l), but not the krt18-expressing gallbladder (g). Red signal anterior to the liver is background staining. (C) Whole-mount right lateral-inferior fluorescent image of 5 dpf wild-type control larva, stained with anti-GFP, showing background autofluorescence in larval tissues. (D) Whole-mount right lateral-inferior fluorescent image of 5 dpf Tg(krt18:egfp) larva stained with anti-GFP, showing EGFP expression in the developing biliary system, skin, and proximal tubule. Abbreviations: l = liver, g = gallbladder, i = intestine, t = trunk, cd = cystic duct, chd = common hepatic duct, cbd = common bile duct, pt = proximal tubule.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage: Day 5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Gene expression patterns : GEP, 14(2), Wilkins, B.J., Gong, W., and Pack, M., A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver, 62-68, Copyright (2014) with permission from Elsevier. Full text @ Gene Expr. Patterns