|ZFIN ID: ZDB-FIG-140421-1|
krt18 and Tg(krt18:egfp) are expressed in the developing biliary system. (A) Whole-mount in situ hybridization of 5 dpf wild-type larva (left lateral view), showing gallbladder expression of krt18 (arrow). (B) Combined immunostaining and fluorescent in situ hybridization of 5 dpf Tg(tp1bglob:egfp) larva (left lateral view), showing Notch-responsive GFP protein in intrahepatic bile ducts within the liver (l), but not the krt18-expressing gallbladder (g). Red signal anterior to the liver is background staining. (C) Whole-mount right lateral-inferior fluorescent image of 5 dpf wild-type control larva, stained with anti-GFP, showing background autofluorescence in larval tissues. (D) Whole-mount right lateral-inferior fluorescent image of 5 dpf Tg(krt18:egfp) larva stained with anti-GFP, showing EGFP expression in the developing biliary system, skin, and proximal tubule. Abbreviations: l = liver, g = gallbladder, i = intestine, t = trunk, cd = cystic duct, chd = common hepatic duct, cbd = common bile duct, pt = proximal tubule.
|EGFP||p314Tg||standard conditions||Day 5||common bile duct||IHC|
|Day 5||cystic duct||IHC|
|Day 5||gall bladder||IHC|
|Day 5||hepatic duct||IHC|
|Day 5||intrahepatic bile duct||IHC|
|Day 5||liver and biliary system||IHC|
|um14Tg/+||standard conditions||Day 5||intrahepatic bile duct||IHC|
|krt18a.1||WT||standard conditions||Day 5||gall bladder||ISH|
|um14Tg/+||standard conditions||Day 5||gall bladder||ISH|
ZFIN wishes to thank the journal Gene expression patterns : GEP for permission to reproduce figures from this article.
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Reprinted from Gene expression patterns : GEP, 14(2), Wilkins, B.J., Gong, W., and Pack, M., A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver, 62-68, Copyright (2014) with permission from Elsevier. Full text @ Gene Expr. Patterns