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ZFIN ID: ZDB-FIG-140421-1
Wilkins et al., 2014 - A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver. Gene expression patterns : GEP   14(2):62-68 Full text @ Gene Expr. Patterns
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5

Fig. 1

krt18 and Tg(krt18:egfp) are expressed in the developing biliary system. (A) Whole-mount in situ hybridization of 5 dpf wild-type larva (left lateral view), showing gallbladder expression of krt18 (arrow). (B) Combined immunostaining and fluorescent in situ hybridization of 5 dpf Tg(tp1bglob:egfp) larva (left lateral view), showing Notch-responsive GFP protein in intrahepatic bile ducts within the liver (l), but not the krt18-expressing gallbladder (g). Red signal anterior to the liver is background staining. (C) Whole-mount right lateral-inferior fluorescent image of 5 dpf wild-type control larva, stained with anti-GFP, showing background autofluorescence in larval tissues. (D) Whole-mount right lateral-inferior fluorescent image of 5 dpf Tg(krt18:egfp) larva stained with anti-GFP, showing EGFP expression in the developing biliary system, skin, and proximal tubule. Abbreviations: l = liver, g = gallbladder, i = intestine, t = trunk, cd = cystic duct, chd = common hepatic duct, cbd = common bile duct, pt = proximal tubule.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP p314Tg standard conditions Day 5 common bile duct IHC
Day 5 cystic duct IHC
Day 5 gall bladder IHC
Day 5 hepatic duct IHC
Day 5 integument IHC
Day 5 intestine IHC
Day 5 intrahepatic bile duct IHC
Day 5 liver IHC
Day 5 liver and biliary system IHC
um14Tg/+ standard conditions Day 5 intrahepatic bile duct IHC
Day 5 liver IHC
krt18a.1 WT standard conditions Day 5 gall bladder ISH
um14Tg/+ standard conditions Day 5 gall bladder ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Gene expression patterns : GEP for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene expression patterns : GEP, 14(2), Wilkins, B.J., Gong, W., and Pack, M., A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver, 62-68, Copyright (2014) with permission from Elsevier. Full text @ Gene Expr. Patterns