FIGURE

Fig. 4

ID

ZDB-FIG-140325-36

Publication

Priyadarshini et al., 2013 - Oxidative stress and regulation of Pink1 in zebrafish (Danio rerio)

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

In-situ hybridization and q-RT-PCR at 3dpf in wild type fish following treatment with H₂O₂ and H₂O₂+LGR.

A. The th2 transcript levels significantly decreased in the H₂O₂ treated samples, verified by q-RT-PCR.

B. The pink1 transcript levels increased significantly in the H₂O₂ treated as compared to wild type samples.

C. No significant change in the th1 transcript levels was detected between the groups. Only a declining trend was observed.

Unpaired t-test (* p-value < 0.05, ** p-value< 0.01).

D-F. The expression of th2 mRNA is reduced in group 10b of the 7-dpf H₂O₂-treated fish as compared with untreated control fish. In the LGR-treated groups at 8 dpf, the decrease in th2 mRNA expression was prevented. The th2-expressing neuron groups 3b, 8b, 9b, 10b are identified according to [26].

Scale bar represents 100 μM. th1 – tyrosine hydroxylase1, th2 – tyrosine hydroxylase 2, pink1 – PTEN-induced putative kinase 1.

Expression Data

Gene:	th2
Fish:	WT
Condition:	chemical treatment: pharmaceutical
Anatomical Term:	dopaminergic neuron
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One