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ZFIN ID: ZDB-FIG-140314-9
Williams et al., 2013 - Developmental expression of the Nfe2-related factor (Nrf) transcription factor family in the zebrafish, Danio rerio. PLoS One   8(10):e79574 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage: Day 4

Fig. 9

Effects of tBOOH and Nrf2a and Nrf2b morpholino treatment on nrf gene expression.

Embryos were injected at the one- to four-cell stage with control-MO, nrf2a-MO, or nrf2b-MO and then treated with either water or 0.5 mM tBOOH for 6 hours starting at 96 hpf. Values were normalized to the control (embryos injected with control-MO and treated with water) within each gene; β-actin 1 (actb1) was used as the housekeeping gene. Fold change data are presented as the mean ± S.E.M. (error bars), and N = 3 pools of 25 embryos. Data were analyzed using ANOVA and Fisher′s PLSD (A, difference between water and tBOOH within a MO treatment; B, difference between control MO with water treatment and nrf2b MO with water treatment; C, difference control MO with tBOOH treatment and either nrf2a or nrf2b MO with tBOOH treatment; p ≤ 0.05).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nfe2 WT control Day 4 whole organism RTPCR
WT chemical treatment: pharmaceutical Day 4 whole organism RTPCR
WT + MO1-nfe2l2a chemical treatment: pharmaceutical Day 4 whole organism RTPCR
WT + MO1-nfe2l2b chemical treatment: pharmaceutical Day 4 whole organism RTPCR
nfe2l1a WT control Day 4 whole organism RTPCR
WT chemical treatment: pharmaceutical Day 4 whole organism RTPCR
WT + MO1-nfe2l2a chemical treatment: pharmaceutical Day 4 whole organism RTPCR
WT + MO1-nfe2l2b chemical treatment: pharmaceutical Day 4 whole organism RTPCR
nfe2l1b WT control Day 4 whole organism RTPCR
WT chemical treatment: pharmaceutical Day 4 whole organism RTPCR
WT + MO1-nfe2l2a chemical treatment: pharmaceutical Day 4 whole organism RTPCR
WT + MO1-nfe2l2b chemical treatment: pharmaceutical Day 4 whole organism RTPCR
nfe2l3 WT control Day 4 whole organism RTPCR
WT chemical treatment: pharmaceutical Day 4 whole organism RTPCR
WT + MO1-nfe2l2a chemical treatment: pharmaceutical Day 4 whole organism RTPCR
WT + MO1-nfe2l2b chemical treatment: pharmaceutical Day 4 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One