header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-140314-2
Williams et al., 2013 - Developmental expression of the Nfe2-related factor (Nrf) transcription factor family in the zebrafish, Danio rerio. PLoS One   8(10):e79574 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: 1-cell to Day 4

Fig. 1

The expression of the nrf gene family during development as measured by quantitative real-time PCR.

Black lines indicate expression pre-hatch and grey lines indicate expression post-hatch. Values were normalized to the 120 hpf time-point and β-actin 1 (actb1) was used as the housekeeping gene. Data are presented as the mean ± S.E.M. (error bars), and N = 4 pools of 30 embryos. Differences in expression between hatching state were assessed using ANOVA followed by Fisher′s PLSD (*, p ≤ 0.05). Data for expression of nrf2a and nrf2b are from Timme-Laragy et al. [7] and are used with permission of the American Society for Biochemistry and Molecular Biology. Previously these data were presented as molecule number. Here we have normalized the data to β-actin 1 expression and to the 120 hpf time point and re-plotted for comparison to the new data on expression of other nrf family genes. Dashed lines indicate the value of 1.0 from the 120 hpf time point.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
nfe2 WT standard conditions 1-cell to Germ-ring whole organism RTPCR
Shield to 26+ somites Not Detected whole organism RTPCR
Prim-5 to Day 4 whole organism RTPCR
nfe2l1a WT standard conditions 1-cell to High-pec whole organism RTPCR
Long-pec to Pec-fin Not Detected whole organism RTPCR
Protruding-mouth to Day 4 whole organism RTPCR
nfe2l1b WT standard conditions Shield to Day 4 whole organism RTPCR
nfe2l2a WT standard conditions 5-9 somites to Day 4 whole organism RTPCR
nfe2l2b WT standard conditions 1-cell to Long-pec whole organism RTPCR
Protruding-mouth to Day 4 whole organism RTPCR
nfe2l3 WT standard conditions 1-cell whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One