FIGURE

Fig. 4

ID

ZDB-FIG-140311-43

Publication

Zhang et al., 2013 - Assessment of hematopoietic failure due to Rpl11 deficiency in a zebrafish model of Diamond-Blackfan anemia by deep sequencing

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Rpl11 deficiency is required for hematopoietic defects through p53-independent pathways in zebrafish embryos. A-C, Rpl11 is required for HSC formation through p53-independent pathways. The expression of cmyb was significantly decreased in Rpl11 morphants and in Rpl11 and p53 double morphants at 48 hpf compared with that in control embryos. D-I, Rpl11 deficiency caused defective hematopoietic phenotypes in zebrafish embryos through p53-independent pathways. Hemoglobin staining of embryos individually injected with Rpl11 MO or Rps19 MO and coinjected with p53 MO were performed. D and G are controls, E and H are Rpl11 MO or Rps19 knockdown, and F and I are Rpl11 MO and Rps19 MO coinjected with p53 MO, respectively. Panel A–C, lateral views; D-I, ventral views.

Expression Data

Gene:	myb
Fish:	WT WT + MO1-rpl11 WT + MO1-rpl11 + MO4-tp53
Knockdown Reagents:	MO1-rpl11 MO4-tp53
Anatomical Term:	hematopoietic stem cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-rpl11 WT + MO1-rpl11 + MO4-tp53 WT + MO1-rps19
Knockdown Reagents:	MO1-rpl11 MO1-rps19 MO4-tp53
Observed In:	hemopoiesis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Genomics