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ZFIN ID: ZDB-FIG-140219-19
Williams et al., 2013 - Developmental expression of the Nfe2-related factor (Nrf) transcription factor family in the zebrafish, Danio rerio. PLoS One   8(10):e79574 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 5

Effects of Nfe2 knockdown on swim bladder inflation and histology.

Thirty larvae were sampled at 96 hpf after being injected as embryos with either control morpholino (A) or nfe2 morpholino (B) and histological analysis was conducted on eight larvae from each treatment.  Normal histology is shown for larvae injected with control MO (C,D).  In larvae with Nfe2 knocked down, cellular disorganization is shown in the mucus cells lining the esophagus (E).  Scale bars represent 25 μm (D) and 30 μm (E). In (C), (D), and (E), cellular structures are numbered as follows: 1: pneumatic duct; 2: notochord; 3: swim bladder; 4: gastrointestinal lumen; 5: yolk; 6: normal columnar epithelium; 7: irregular, disordered epithelium lacking well developed mucus cells; 8: irregular epithelium. Anterior (A), posterior (P), dorsal (D), and ventral (V) are also labeled.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-nfe2 standard conditions Day 4 esophageal epithelium mucus secreting cell decreased amount, abnormal
Day 4 esophagus muscle decreased size, abnormal
Day 4 gill morphology, abnormal
Day 4 pharyngeal arch morphology, abnormal
Day 4 pneumatic duct epithelial cell decreased amount, abnormal
Day 4 pneumatic duct epithelial cell disoriented, abnormal
Day 4 pneumatic duct epithelial cell increased variability of size, abnormal
Day 4 pneumatic duct epithelium morphology, abnormal
Day 4 swim bladder uninflated, abnormal
WT + MO2-nfe2 standard conditions Day 4 esophageal epithelium mucus secreting cell decreased amount, abnormal
Day 4 esophagus muscle decreased size, abnormal
Day 4 gill morphology, abnormal
Day 4 pharyngeal arch morphology, abnormal
Day 4 pneumatic duct epithelial cell decreased amount, abnormal
Day 4 pneumatic duct epithelial cell disoriented, abnormal
Day 4 pneumatic duct epithelial cell increased variability of size, abnormal
Day 4 pneumatic duct epithelium morphology, abnormal
Day 4 swim bladder uninflated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One