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ZIRC
ZFIN ID: ZDB-FIG-140214-37
Valvezan et al., 2014 - Oncogenic mutations in Adenomatous Polyposis Coli (Apc) activate mechanistic Target of Rapamycin Complex 1 (mTORC1). Disease models & mechanisms   7(1):63-71 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 1

Activation of mTORC1 in apcmcr/mcr zebrafish. Western blot analysis using antibodies to phosphorylated S6 (p-S6), indicating mTORC1 activity, or total S6 on whole embryo lysates from pooled wild-type and heterozygous apcmcr/+ zebrafish or homozygous apcmcr/mcr zebrafish at 3 or 4 dpf. mTORC1 activity was increased in the homozygous apcmcr/mcr zebrafish. (B) Immunohistochemical staining of sagittal sections from wild-type or homozygous apcmcr/mcr zebrafish at 3 dpf using antibodies to phosphorylated or total S6 and counterstaining with DAPI. mTORC1 was aberrantly active in multiple mesodermal and endodermal derivatives in the homozygous apcmcr/mcr zebrafish.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-rps6 IHC apchu745/hu745 standard conditions Protruding-mouth endoderm
IHC Protruding-mouth mesodermal cell
Ab8-rps6 IHC apchu745/hu745 standard conditions Protruding-mouth endoderm
IHC Protruding-mouth mesodermal cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.